Lüth H J, Blümcke I, Winkelmann E, Celio M R
Paul-Flechsig-Institute of Brain Research, Dept. of Neuroanatomy, University of Leipzig.
J Hirnforsch. 1993;34(1):93-103.
The distribution of the calcium binding protein, calretinin (CR) has been investigated immunohistochemically in the cerebral cortex of albino rats by light- and electron microscopy. At the light microscopical level the pattern of CR-immunoreactivity (ir) appeared very similar in all regions of the rat cerebral cortex. CR-immunoreactive cells were found sparsely in layer I to layer VI, and frequently also in the white matter of the corpus callosum. All CR-ir neurons revealed morphological characteristics of local interneurons. The calretinin positive interneurons could be grouped according to their laminar occurrence, dendritic arborization and the soma size into 5 cell type classes. Quantitative measurements were performed only in the visual cortex. CR-ir neurons were more frequent in the superficial layers II and III. In all other layers, CR-ir cells are sparsely distributed with no preferential laminar localization. At the electron microscopical level, CR-ir axonal boutons formed frequently symmetrical axo-dendritic contacts. In all animals we observed CR-ir axons forming also synaptses of asymmetrical type. In summary calretinin labelled an interneuronal subpopulation of the rat cerebral cortex, which seemed not to overlap in its distribution and labelled structures to those, containing the related calcium binding proteins parvalbumin and calbindin D-28k.
通过光学显微镜和电子显微镜，采用免疫组织化学方法对白化大鼠大脑皮质中钙结合蛋白钙视网膜蛋白（CR）的分布进行了研究。在光学显微镜水平上，大鼠大脑皮质所有区域的CR免疫反应性（ir）模式看起来非常相似。在I层至VI层中稀疏地发现了CR免疫反应性细胞，在胼胝体白质中也经常发现。所有CR免疫反应性神经元均显示出局部中间神经元的形态特征。钙视网膜蛋白阳性中间神经元可根据其层位分布、树突分支和胞体大小分为5种细胞类型。仅在视觉皮质进行了定量测量。CR免疫反应性神经元在浅层II和III层中更为常见。在所有其他层中，CR免疫反应性细胞分布稀疏，没有优先的层位定位。在电子显微镜水平上，CR免疫反应性轴突终扣经常形成对称的轴突-树突接触。在所有动物中，我们还观察到CR免疫反应性轴突形成不对称类型的突触。总之，钙视网膜蛋白标记了大鼠大脑皮质中间神经元的一个亚群，其分布和标记结构似乎与含有相关钙结合蛋白小白蛋白和钙结合蛋白D-28k的那些不重叠。
