Phillips C, Barrett M P, Gover S, Le Page R W, Adams M J
Laboratory of Molecular Biophysics, Oxford University, U.K.
J Mol Biol. 1993 Sep 20;233(2):317-21. doi: 10.1006/jmbi.1993.1510.
We have obtained well-ordered single crystals of 6-phosphogluconate dehydrogenase, an enzyme of the oxidative branch of the pentose phosphate pathway, from Trypanosoma brucei. The crystals are trigonal rhombs with unit cell dimensions a = b = 135.1 A, c = 116.7 A and belong to one of the enantiomorphic pair of space groups P3(1)21/P3(2)21. X-ray diffraction to better than 2.8 A has been recorded using a rotating anode CuK alpha source. Elucidation of the three-dimensional structure of the T. brucei enzyme will, by indicating structural differences from the known sheep enzyme structure, aid the design of mutants to probe the active site. Knowledge of the structure will also assist in assessing the potential use of rationally designed compounds to inhibit this enzyme specifically.
我们从布氏锥虫中获得了6-磷酸葡萄糖酸脱氢酶（磷酸戊糖途径氧化分支中的一种酶）的有序单晶。这些晶体为三角菱形，晶胞参数a = b = 135.1 Å，c = 116.7 Å，属于空间群P3(1)21/P3(2)21这一对对映形态之一。使用旋转阳极CuKα源记录了分辨率优于2.8 Å的X射线衍射数据。阐明布氏锥虫该酶的三维结构，通过揭示其与已知绵羊酶结构的差异，将有助于设计突变体以探测活性位点。该结构的知识也将有助于评估合理设计的化合物特异性抑制这种酶的潜在用途。
