Masana Y, Wanaka A, Kato H, Asai T, Tohyama M
Department of Anatomy and Neuroscience, Osaka University Medical School, Japan.
J Neurosci Res. 1993 Aug 1;35(5):468-79. doi: 10.1002/jnr.490350503.
We investigated the localization of trkB mRNA, which encodes a putative component of high-affinity brain-derived neurotrophic factor (BDNF) or the neurotrophin-3 (NT-3) receptor, in the postnatal rat brain by in situ hybridization histochemistry. At birth, trkB mRNA was strongly expressed in various regions with the thalamus and cerebral cortex showing the strongest expression. As the rat grows, expression generally persisted or declined in most regions with the exception of the hippocampus where trkB mRNA expression increased during postnatal development. In the adult brain, trkB mRNA was detected in the olfactory system, cerebral cortex, hippocampal formation, amygdala, and cerebellar cortex. These findings, together with the developmental profiles of BDNF and NT-3 mRNA expressions, suggest that trkB product (gp145trkB) mainly transduces NT-3 signals early in the postnatal period, and BDNF signals later in the period.
我们通过原位杂交组织化学方法，研究了编码高亲和力脑源性神经营养因子（BDNF）或神经营养因子-3（NT-3）受体假定成分的trkB mRNA在出生后大鼠脑中的定位。出生时，trkB mRNA在各个区域强烈表达，其中丘脑和大脑皮层表达最强。随着大鼠生长，除海马体外，大多数区域的表达通常持续或下降，而海马体中trkB mRNA表达在出生后发育过程中增加。在成体脑中，在嗅觉系统、大脑皮层、海马结构、杏仁核和小脑皮层中检测到trkB mRNA。这些发现，连同BDNF和NT-3 mRNA表达的发育情况，表明trkB产物（gp145trkB）在出生后早期主要转导NT-3信号，后期转导BDNF信号。
