Lewin E, Ladefoged J, Brandi L, Olgaard K
Nephrological Dept. P, Rigshospitalet, University of Copenhagen, Denmark.
Kidney Int. 1993 Aug;44(2):379-84. doi: 10.1038/ki.1993.255.
Chronic renal failure (CRF) is combined with an impairment of the immune system. The T cell may be a target for the action of parathyroid hormone (PTH). Rats with CRF have high blood levels of PTH. Therefore, the present investigation examined some aspects of the T cell function in both normal and CRF rats before and after parathyroidectomy and after an isogenic kidney transplantation. The T cell proliferative response to phytohemagglutinin (PHA) stimulation was significantly higher in peripheral blood mononuclear cell (PBMC) cultures obtained from CRF rats than from normal rats. After parathyroidectomy the T cells of normal as well as of uremic rats could still be significantly stimulated by PHA, but now no significant difference was seen. When CRF was reversed after an isogenic kidney transplantation and PTH reversed to levels in the normal range, the T cell proliferative response to PHA was normalized. Rat PTH 1-84 stimulated in vitro the PHA-induced proliferation of T cells in a dose dependent manner. This effect was significant in CRF rat lymphocytes, but not in lymphocytes obtained from normal rats. Based upon the present results it is suggested that the secondary hyperparathyroidism in chronic uremia is responsible for the enhanced proliferative response to PHA of T cells from CRF rats.
慢性肾衰竭（CRF）常伴有免疫系统损害。T细胞可能是甲状旁腺激素（PTH）作用的靶点。CRF大鼠的血液中PTH水平较高。因此，本研究检测了正常大鼠和CRF大鼠在甲状旁腺切除术前、术后以及同基因肾移植后的T细胞功能的某些方面。从CRF大鼠获得的外周血单核细胞（PBMC）培养物中，T细胞对植物血凝素（PHA）刺激的增殖反应显著高于正常大鼠。甲状旁腺切除术后，正常大鼠和尿毒症大鼠的T细胞仍能被PHA显著刺激，但此时未观察到显著差异。当同基因肾移植后CRF得到逆转且PTH恢复到正常范围时，T细胞对PHA的增殖反应也恢复正常。大鼠PTH 1-84以剂量依赖的方式在体外刺激T细胞的PHA诱导增殖。这种作用在CRF大鼠淋巴细胞中显著，但在正常大鼠淋巴细胞中不显著。基于目前的结果，提示慢性尿毒症中的继发性甲状旁腺功能亢进是CRF大鼠T细胞对PHA增殖反应增强的原因。
