Watanabe K, Hayashi M, Senga S, Miyata T, Iida T, Onitsuka A, Hirose H
First Department of Surgery, Gifu University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1993 Aug;94(8):796-800.
This study was focused on the changes of high energy phosphates and tissue blood flow (TBF) in ischemia and reperfusion, of jaundiced rat liver. We ligated and cut the bile duct of male Wistar rats to induce jaundice. The rats were divided into two groups, BDL-4W which had ligation four weeks before examination and control which had no ligation. Two studies were performed; In study A, ischemia was induced by clamping portal vein and hepatic artery for 60 minutes. In study B, 15 minutes of ischemia was repeated four times with intervals of 10 minutes of reperfusion. A: 1) In BDL-4W, ATP was significantly lower than that in control at pre-ischemia and reperfusion. Recovery ratios (RR) of ATP in BDL-4W were significantly lower than those in control. 2) RR or TBF in BDL-4W were significantly lower than those in control. 3) In BDL-4W, xanthine was significantly higher than that in control at reperfusion. B: 4) There were no significant differences in the RR of ATP after reperfusion between two groups. 5) There were no significant differences in the RR of TBF between two groups. 6) There were no significant differences in xanthine after reperfusion between two groups. We concluded that intermittent brief ischemia was useful when 60 minutes of ischemia was required for the operation of jaundiced liver.
本研究聚焦于黄疸大鼠肝脏缺血再灌注过程中高能磷酸化物和组织血流量（TBF）的变化。我们结扎并切断雄性Wistar大鼠的胆管以诱导黄疸。将大鼠分为两组，BDL - 4W组在检查前四周进行结扎，对照组未进行结扎。进行了两项研究；在研究A中，通过夹闭门静脉和肝动脉60分钟诱导缺血。在研究B中，15分钟的缺血重复四次，每次缺血间隔10分钟的再灌注。A：1）在BDL - 4W组中，缺血前和再灌注时ATP显著低于对照组。BDL - 4W组中ATP的恢复率（RR）显著低于对照组。2）BDL - 4W组中RR或TBF显著低于对照组。3）在BDL - 4W组中，再灌注时黄嘌呤显著高于对照组。B：4）两组再灌注后ATP的RR无显著差异。5）两组TBF的RR无显著差异。6）两组再灌注后黄嘌呤无显著差异。我们得出结论，当黄疸肝脏手术需要60分钟缺血时，间歇性短暂缺血是有用的。
