[Changes in high energy phosphates and tissue blood flow in ischemia and reperfusion of jaundiced rat liver--effect of intermittent brief ischemia].

This study was focused on the changes of high energy phosphates and tissue blood flow (TBF) in ischemia and reperfusion, of jaundiced rat liver. We ligated and cut the bile duct of male Wistar rats to induce jaundice. The rats were divided into two groups, BDL-4W which had ligation four weeks before examination and control which had no ligation. Two studies were performed; In study A, ischemia was induced by clamping portal vein and hepatic artery for 60 minutes. In study B, 15 minutes of ischemia was repeated four times with intervals of 10 minutes of reperfusion. A: 1) In BDL-4W, ATP was significantly lower than that in control at pre-ischemia and reperfusion. Recovery ratios (RR) of ATP in BDL-4W were significantly lower than those in control. 2) RR or TBF in BDL-4W were significantly lower than those in control. 3) In BDL-4W, xanthine was significantly higher than that in control at reperfusion. B: 4) There were no significant differences in the RR of ATP after reperfusion between two groups. 5) There were no significant differences in the RR of TBF between two groups. 6) There were no significant differences in xanthine after reperfusion between two groups. We concluded that intermittent brief ischemia was useful when 60 minutes of ischemia was required for the operation of jaundiced liver.