Mori M, Barnard G F, Staniunas R J, Jessup J M, Steele G D, Chen L B
Department of Surgery, New England Deaconess Hospital, Boston, Massachusetts.
Oncogene. 1993 Oct;8(10):2821-6.
Prothymosin alpha (PT-alpha) is a nuclear protein involved in cell proliferation. Transcription of PT-alpha has been reported to be regulated by the c-myc gene in vitro. We identified PT-alpha as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-alpha and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-alpha and c-myc supported these findings. There was no correlation between PT-alpha or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-alpha expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-alpha gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.
前胸腺素α（PT-α）是一种参与细胞增殖的核蛋白。据报道，PT-α的转录在体外受c-myc基因调控。我们在一个人结肠癌减去正常黏膜的消减cDNA文库中鉴定出PT-α过表达。Northern印迹（信使RNA）分析表明，与相邻正常组织相比，PT-α和c-myc基因在人结肠直肠癌中均过表达。对PT-α和c-myc的免疫组织化学研究支持了这些发现。PT-α或c-myc信使RNA表达与结直肠癌的Dukes分期之间没有相关性；这两者与肌动蛋白信使RNA表达之间也没有相关性。然而，PT-α表达与c-myc表达之间存在显著相关性（P < 0.001）。这些发现支持了这样一种假说，即PT-α基因转录可能与人类结肠直肠癌中的c-myc基因相关，并且可能受其控制。
