Weilenmann R, Arnold S, Döbeli M, Rüsch P, Zerobin K
Division Pharma, Forschung und Entwicklung, CIBA-GEIGY AG, Basel.
Schweiz Arch Tierheilkd. 1993;135(8):236-41.
Oestrus was induced in 14 anoestrous beagle bitches by intramuscular injection of PMSG in a dose of 20 I.U./kg once daily on five consecutive days, followed by an additional single i.m. injection of 500 I.U. of hCG on the fifth day. The day on which the first injection was given was counted as Day 1 of the experiment. Between the fourth and sixth day, the bitches began to attract the attention of male dogs and between Days 9 and 15 all bitches came in heat. Matings occurred on two to ten occasions, and six of the bitches conceived. The maximum concentrations of oestradiol in the plasma were in most cases reached on Days 10 or 12 and ranged from 42 to 195 pg/ml. In all cases progesterone concentrations rose sharply between Days 10 and 20. The incretion phase of the corpora lutea was noticeably brief in the non-gravid bitches; in five of these eight bitches, anoestrous values below 2 ng/ml were already obtained before the 65th day of the experiment. These results indicate that the administration of PMSG on five consecutive days supplemented by a final single injection of hCG stimulates the ovaries adequately to afford good prospects of conception. The concentrations of ovarian hormones are discussed.
通过连续五天每天一次肌肉注射剂量为20国际单位/千克的孕马血清促性腺激素（PMSG），诱导14只处于乏情期的比格犬发情，随后在第五天额外单次肌肉注射500国际单位的人绒毛膜促性腺激素（hCG）。首次注射的当天被计为实验的第1天。在第4天至第6天之间，母犬开始引起公犬的注意，在第9天至第15天之间，所有母犬都发情。交配发生了两到十次，其中六只母犬受孕。血浆中雌二醇的最高浓度在大多数情况下在第10天或第12天达到，范围为42至195皮克/毫升。在所有情况下，孕酮浓度在第10天至第20天之间急剧上升。未受孕母犬黄体的分泌期明显短暂；在这八只母犬中的五只中，在实验的第65天之前就已经获得了低于2纳克/毫升的乏情期值。这些结果表明，连续五天给予PMSG并辅以最后一次单次注射hCG能充分刺激卵巢，从而提供良好的受孕前景。文中还讨论了卵巢激素的浓度。
