表皮生长因子（EGF）和γ干扰素对小鼠肝细胞核中酪氨酸磷酸化DNA结合蛋白的诱导作用

Induction by EGF and interferon-gamma of tyrosine phosphorylated DNA binding proteins in mouse liver nuclei.

作者信息

Ruff-Jamison S, Chen K, Cohen S

机构信息

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.

出版信息

Science. 1993 Sep 24;261(5129):1733-6. doi: 10.1126/science.8378774.

DOI:10.1126/science.8378774

PMID:8378774

Abstract

Intraperitoneal injection of epidermal growth factor (EGF) into mice resulted in the appearance in liver nuclei of three tyrosine phosphorylated proteins (84, 91, and 92 kilodaltons) within minutes after administration of EGF. Administration of interferon-gamma (IFN-gamma) resulted in the appearance in liver nuclei of two tyrosine phosphorylated proteins (84 and 91 kilodaltons). The 84- and 91-kilodalton proteins detected after either EGF or IFN-gamma administration were identified as the IFN-gamma activation factors (GAF). Furthermore, gel shift analysis revealed that these GAF proteins, detected after either EGF or IFN-gamma administration, specifically bound to the sis-inducible element of the c-fos promoter. Thus, GAF proteins participate in nuclear signaling in both IFN-gamma and EGF pathways.

摘要

给小鼠腹腔注射表皮生长因子（EGF）后，在注射EGF数分钟内，肝脏细胞核中出现了三种酪氨酸磷酸化蛋白（84、91和92千道尔顿）。注射γ干扰素（IFN-γ）后，肝脏细胞核中出现了两种酪氨酸磷酸化蛋白（84和91千道尔顿）。在注射EGF或IFN-γ后检测到的84千道尔顿和91千道尔顿蛋白被鉴定为IFN-γ激活因子（GAF）。此外，凝胶迁移分析表明，在注射EGF或IFN-γ后检测到的这些GAF蛋白特异性结合到c-fos启动子的sis诱导元件上。因此，GAF蛋白参与了IFN-γ和EGF信号通路中的核信号传导。