Gavella M, Lipovac V
Vuk Vrhovac Institute for Diabetes, Endocrinology and Metabolic Diseases, School of Medicine, University of Zagreb, Croatia.
Arch Androl. 1993 Jan-Feb;30(1):55-61. doi: 10.3109/01485019308988369.
The hypoosmotic swelling test was used to evaluate the size of physiologically competent sperm after their exposure to the influence of leucocyte-derived superoxide anions. In sperm suspensions, preincubated in the presence of 50 x 10(4)mL-1 leucocytes capable of producing 0.93 +/- 0.63 nmol of O2- (range 0.21-2.03 nmol) over 30 min at 37 degrees C, a significant decrease in the percentage of swollen sperm compared to that in the control sperm suspensions without added leucocytes was found (p < .001, n = 46). Addition of phorbol myristate acetate, an activator of the release of O2-, further diminished the ability of hypoosmotic sperm tail swelling (p < .001), while superoxide dismutase inhibited this effect. This study suggests that even very low amounts of superoxide anions may influence the functional integrity of sperm. Therefore, any source of superoxide anion production should be eliminated and/or inhibited in sperm suspensions used in assisted reproduction techniques.
低渗肿胀试验用于评估生理活性精子在受到白细胞衍生的超氧阴离子影响后的大小。在精子悬液中，预先在50×10⁴个/mL能够在37℃下30分钟产生0.93±0.63 nmol O₂⁻（范围为0.21 - 2.03 nmol）的白细胞存在下孵育，与未添加白细胞的对照精子悬液相比，发现肿胀精子的百分比显著降低（p < 0.001，n = 46）。添加佛波醇肉豆蔻酸酯（一种O₂⁻释放的激活剂）进一步降低了低渗精子尾部肿胀的能力（p < 0.001），而超氧化物歧化酶抑制了这种作用。这项研究表明，即使是非常少量的超氧阴离子也可能影响精子的功能完整性。因此，在辅助生殖技术中使用的精子悬液中，应消除和/或抑制任何超氧阴离子产生的来源。
