菠菜叶中编码核苷二磷酸激酶II的cDNA的核苷酸序列。

Nucleotide sequence of the cDNA encoding nucleoside diphosphate kinase II from spinach leaves.

作者信息

Zhang J, Nomura T, Yatsunami K, Honda A, Sugimoto Y, Moriwaki T, Yamamoto J, Ohta M, Fukui T, Ichikawa A

机构信息

Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.

出版信息

Biochim Biophys Acta. 1993 Jan 23;1171(3):304-6. doi: 10.1016/0167-4781(93)90070-t.

DOI:10.1016/0167-4781(93)90070-t

PMID:8381027

Abstract

The primary structure of nucleoside diphosphate (NDP) kinase II, one of the two isozymes found in spinach leaves, has been deduced from its cDNA sequence. NDP kinase II comprises 233 amino acid residues and has a molecular mass of 26,107 Da, which is larger than that of the purified NDP kinase II subunits (18 kDa) by about 8 kDa, suggesting that NDP kinase II might be post-translationally processed. Homology was found between the sequence of spinach NDP kinase II, and the sequences of spinach NDP kinase I, rat NDP kinases alpha and beta, Dictyostelium discoideum NDP kinase, the human Nm23-H1 and Nm23-H2 proteins and the awd protein of Drosophila melanogaster.

摘要

核苷二磷酸（NDP）激酶II是在菠菜叶中发现的两种同工酶之一，其一级结构已根据其cDNA序列推导得出。NDP激酶II由233个氨基酸残基组成，分子量为26,107 Da，比纯化的NDP激酶II亚基（18 kDa）大约大8 kDa，这表明NDP激酶II可能经过了翻译后加工。在菠菜NDP激酶II的序列与菠菜NDP激酶I、大鼠NDP激酶α和β、盘基网柄菌NDP激酶、人类Nm23-H1和Nm23-H2蛋白以及果蝇的awd蛋白的序列之间发现了同源性。