Warters R L, Chu G L, Wong R S, Lyons B W, Dewey W C
Department of Radiology, University of Utah Health Sciences Center, Salt Lake City 84132.
J Cell Physiol. 1993 Feb;154(2):402-9. doi: 10.1002/jcp.1041540224.
An increase was observed in the total protein mass of nuclei isolated from Chinese hamster ovary cells heated at 45 degrees C or 45.5 degrees C. An increase in the fractional recovery of DNA polymerase alpha and beta, and of DNA topoisomerase activity coincided with this increase in the protein mass of nuclei from heated cells. Nuclear protein mass which was soluble in 2.0 M NaCl decreased 0.5 fold, while DNA-associated and nuclear matrix-associated protein mass increased 2.2 and 3.4 fold, respectively. The results indicate that the increase in nuclear protein mass observed in nuclei from heated cells is due in part to an increased binding, or precipitation, of nuclear proteins onto the cell's DNA and nuclear matrix.
在从45摄氏度或45.5摄氏度加热的中国仓鼠卵巢细胞中分离出的细胞核中，观察到总蛋白质量有所增加。DNA聚合酶α和β以及DNA拓扑异构酶活性的部分回收率增加，这与加热细胞的细胞核蛋白质量增加相一致。可溶于2.0 M氯化钠的核蛋白质量减少了0.5倍，而与DNA相关的和与核基质相关的蛋白质量分别增加了2.2倍和3.4倍。结果表明，在加热细胞的细胞核中观察到的核蛋白质量增加部分是由于核蛋白与细胞DNA和核基质的结合或沉淀增加所致。
