用亲和色谱法从人子宫中纯化雌二醇受体。
Purification of oestradiol receptor from human uterus by affinity chromotrgraphy.
作者信息
Coffer A I, Milton P J, Pryse-Davies J, King R J
出版信息
Mol Cell Endocrinol. 1977 Feb;6(4-5):231-46. doi: 10.1016/0303-7207(77)90098-3.
The oestrogen receptor from human myometrium has been extensively purified by affinity chromatography and isoelectric focusing. The latter step is necessary to remove contaminating sex-steroid-binding globulin. The unpurified 3.7-S cytoplasmic receptor has a molecular weight of 41,000, a Stokes radius of 27.0 A and a frictional ratio (f/f0) of 1.19; the KD (4 degrees C) for [3H]oestradiol-17 beta was 1.03 X 10(-10) M. After purification, the molecular weight was 30,000, the Stokes radius 23.6 A, frictional ratio 1.15 and isoelectric point 6.15.
人子宫肌层的雌激素受体已通过亲和层析和等电聚焦进行了广泛纯化。后一步骤对于去除污染的性甾体结合球蛋白是必要的。未纯化的3.7-S细胞质受体的分子量为41,000，斯托克斯半径为27.0 Å，摩擦比（f/f0）为1.19；[3H]雌二醇-17β在4℃时的解离常数（KD）为1.03×10^(-10) M。纯化后，分子量为30,000，斯托克斯半径为23.6 Å，摩擦比为1.15，等电点为6.15。
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