• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自黄色粘球菌的核苷二磷酸激酶的自磷酸化作用。

Autophosphorylation of nucleoside diphosphate kinase from Myxococcus xanthus.

作者信息

Muñoz-Dorado J, Almaula N, Inouye S, Inouye M

机构信息

Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Rutgers, Piscataway 08854.

出版信息

J Bacteriol. 1993 Feb;175(4):1176-81. doi: 10.1128/jb.175.4.1176-1181.1993.

DOI:10.1128/jb.175.4.1176-1181.1993
PMID:8381783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193035/
Abstract

The nucleoside diphosphate kinase (NDP kinase) from Myxococcus xanthus has been purified to homogeneity and crystallized (J. Munoz-Dorado, M. Inouye, and S. Inouye, J. Biol. Chem. 265:2702-2706, 1990). In the presence of ATP, the NDP kinase was autophosphorylated. Phosphoamino acid analysis was carried out after acid and base hydrolyses of phosphorylated NDP kinase. It was found that the protein was phosphorylated not only at a histidine residue but also at a serine residue. Replacement of histidine 117 with a glutamine residue completely abolished the autophosphorylation and nucleotide-binding activity of the NDP kinase. Since histidine 117 is the only histidine residue that is conserved in all known NDP kinases so far characterized, the results suggest that the phosphohistidine intermediate is formed at this residue during the transphosphorylation reaction from nucleoside triphosphates to nucleoside diphosphates. Preliminary mutational analysis of putative ATP-binding sites is also presented.

摘要

黄色粘球菌的核苷二磷酸激酶（NDP激酶）已被纯化至同质并结晶（J. Munoz-Dorado、M. Inouye和S. Inouye，《生物化学杂志》265:2702 - 2706，1990年）。在ATP存在的情况下，NDP激酶发生自身磷酸化。对磷酸化的NDP激酶进行酸碱水解后进行磷酸氨基酸分析。发现该蛋白质不仅在组氨酸残基处磷酸化，而且在丝氨酸残基处也磷酸化。用谷氨酰胺残基替换组氨酸117完全消除了NDP激酶的自身磷酸化和核苷酸结合活性。由于组氨酸117是迄今为止所有已知NDP激酶中唯一保守的组氨酸残基，结果表明在从核苷三磷酸到核苷二磷酸的转磷酸化反应过程中，磷酸组氨酸中间体在该残基处形成。还展示了对假定ATP结合位点的初步突变分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/bf4bf030440a/jbacter00046-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/0fe1dc77ec04/jbacter00046-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/7043839c6dcf/jbacter00046-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/bf4bf030440a/jbacter00046-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/0fe1dc77ec04/jbacter00046-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/7043839c6dcf/jbacter00046-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8d5/193035/bf4bf030440a/jbacter00046-0271-a.jpg

相似文献

1
Autophosphorylation of nucleoside diphosphate kinase from Myxococcus xanthus.来自黄色粘球菌的核苷二磷酸激酶的自磷酸化作用。
J Bacteriol. 1993 Feb;175(4):1176-81. doi: 10.1128/jb.175.4.1176-1181.1993.
2
Autophosphorylation of nucleoside diphosphate kinase on non-histidine residues.
FEBS Lett. 1994 Oct 10;353(1):5-8. doi: 10.1016/0014-5793(94)00997-x.
3
Nucleoside diphosphate kinase from Escherichia coli.来自大肠杆菌的核苷二磷酸激酶
J Bacteriol. 1995 May;177(9):2524-9. doi: 10.1128/jb.177.9.2524-2529.1995.
4
Nucleoside diphosphate kinase from Myxococcus xanthus. II. Biochemical characterization.
J Biol Chem. 1990 Feb 15;265(5):2707-12.
5
Discrimination between acid and alkali-labile phosphorylated residues on Immobilon: phosphorylation studies of nucleoside diphosphate kinase.在Immobilon上区分酸不稳定和碱不稳定的磷酸化残基：核苷二磷酸激酶的磷酸化研究
Anal Biochem. 1996 Nov 15;242(2):165-71. doi: 10.1006/abio.1996.0449.
6
Structure-based mutational and functional analysis identify human NM23-H2 as a multifunctional enzyme.基于结构的突变和功能分析确定人类NM23-H2为一种多功能酶。
Biochemistry. 2002 May 21;41(20):6330-7. doi: 10.1021/bi025606+.
7
A fluorescent sensor of the phosphorylation state of nucleoside diphosphate kinase and its use to monitor nucleoside diphosphate concentrations in real time.一种核苷二磷酸激酶磷酸化状态的荧光传感器及其用于实时监测核苷二磷酸浓度的用途。
Biochemistry. 2001 Apr 24;40(16):5087-94. doi: 10.1021/bi002484h.
8
Characterization of nucleoside-diphosphate kinase from Pseudomonas aeruginosa: complex formation with succinyl-CoA synthetase.铜绿假单胞菌核苷二磷酸激酶的特性：与琥珀酰辅酶A合成酶的复合物形成
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5883-7. doi: 10.1073/pnas.91.13.5883.
9
Putative functions of nucleoside diphosphate kinase in plants and fungi.核苷二磷酸激酶在植物和真菌中的假定功能。
J Bioenerg Biomembr. 2003 Feb;35(1):57-65. doi: 10.1023/a:1023493823368.
10
The crystal structure of a human nucleoside diphosphate kinase, NM23-H2.人核苷二磷酸激酶NM23-H2的晶体结构
J Mol Biol. 1995 Aug 25;251(4):574-87. doi: 10.1006/jmbi.1995.0457.

引用本文的文献

1
Ndk, a novel host-responsive regulator, negatively regulates bacterial virulence through quorum sensing in Pseudomonas aeruginosa.Ndk 是一种新型的宿主反应调节剂，通过铜绿假单胞菌的群体感应负调控细菌毒力。
Sci Rep. 2016 Jun 27;6:28684. doi: 10.1038/srep28684.
2
Phosphoproteomic analysis reveals the effects of PilF phosphorylation on type IV pilus and biofilm formation in Thermus thermophilus HB27.磷酸化蛋白质组学分析揭示了 PilF 磷酸化对嗜热栖热菌 HB27 中 IV 型菌毛和生物膜形成的影响。
Mol Cell Proteomics. 2013 Oct;12(10):2701-13. doi: 10.1074/mcp.M113.029330. Epub 2013 Jul 4.
3
Histidine 117 in the His-Gly-Ser-Asp motif is Required for the Biochemical Activities of Nucleoside Diphosphate Kinase of Mycobacterium smegmatis.

本文引用的文献

1
Some reactions of adenosine and inosine phosphates in animal tissues.动物组织中腺苷酸和肌苷酸的一些反应。
Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):172-80. doi: 10.1016/0006-3002(53)90136-x.
2
Transphosphorylation between nucleoside polyphosphates.核苷多磷酸之间的转磷酸化作用。
Nature. 1953 Nov 28;172(4387):1008-9. doi: 10.1038/1721008a0.
3
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
耻垢分枝杆菌核苷二磷酸激酶的生化活性需要His-Gly-Ser-Asp基序中的组氨酸117。
Open Biochem J. 2012;6:71-7. doi: 10.2174/1874091X01206010071. Epub 2012 Jul 27.
4
Heat shock modulates phosphorylation status and activity of nucleoside diphosphate kinase in cultured sugarcane cells.热激调节培养甘蔗细胞中核苷二磷酸激酶的磷酸化状态和活性。
Plant Cell Rep. 2010 Nov;29(11):1305-14. doi: 10.1007/s00299-010-0917-6. Epub 2010 Sep 7.
5
Emerging roles for protein histidine phosphorylation in cellular signal transduction: lessons from the islet beta-cell.蛋白质组氨酸磷酸化在细胞信号转导中的新作用：来自胰岛β细胞的经验教训。
J Cell Mol Med. 2008 Oct;12(5B):1885-908. doi: 10.1111/j.1582-4934.2008.00330.x. Epub 2008 Apr 8.
6
A modular minimal cell model: purine and pyrimidine transport and metabolism.一种模块化最小细胞模型：嘌呤和嘧啶的转运与代谢。
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6681-6. doi: 10.1073/pnas.0400962101. Epub 2004 Apr 16.
7
Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity.来自嗜热栖热放线菌的开放阅读框sso2387编码一种具有蛋白质丝氨酸激酶活性的多肽。
J Bacteriol. 2003 Jun;185(11):3436-45. doi: 10.1128/JB.185.11.3436-3445.2003.
8
Role of AWD/nucleoside diphosphate kinase in Drosophila development.AWD/核苷二磷酸激酶在果蝇发育中的作用。
J Bioenerg Biomembr. 2000 Jun;32(3):293-300. doi: 10.1023/a:1005545214937.
9
Metabolic functions of microbial nucleoside diphosphate kinases.微生物核苷二磷酸激酶的代谢功能
J Bioenerg Biomembr. 2000 Jun;32(3):259-67. doi: 10.1023/a:1005537013120.
10
Mutational analysis of nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of critical amino acid residues involved in exopolysaccharide alginate synthesis.铜绿假单胞菌核苷二磷酸激酶的突变分析：参与胞外多糖藻酸盐合成的关键氨基酸残基的特性研究
J Bacteriol. 1996 Dec;178(24):7120-8. doi: 10.1128/jb.178.24.7120-7128.1996.
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
4
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.劳氏肉瘤病毒的转化基因产物使酪氨酸磷酸化。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5. doi: 10.1073/pnas.77.3.1311.
5
Preparation of crystalline nucleoside diphosphate kinase from baker's yeast and identification of 1-[32P]phosphohistidine as the main phosphorylated product of an alkaline hydrolysate of enzyme incubated with adenosine [32P]triphosphate.
Eur J Biochem. 1969 Jul;9(4):451-5. doi: 10.1111/j.1432-1033.1969.tb00630.x.
6
Identification of a phosphate-incorporating protein from bovine liver as nucleoside diphosphate kinase and isolation of 1-32P-phosphohistidine, 3-32P-phosphohistidine, and N-epsilon-32P-phospholysine from erythrocytic nucleoside diphosphate kinase, incubated with adenosine triphosphate-32P.从牛肝中鉴定出一种作为核苷二磷酸激酶的含磷蛋白，并从与三磷酸腺苷 - ³²P 一起孵育的红细胞核苷二磷酸激酶中分离出 1 - ³²P - 磷酸组氨酸、3 - ³²P - 磷酸组氨酸和 N - ε - ³²P - 磷酸赖氨酸。
J Biol Chem. 1968 Jul 25;243(14):3947-52.
7
Purification and properties of Bacillus subtilis nucleoside diphosphokinase.枯草芽孢杆菌核苷二磷酸激酶的纯化及性质
J Biol Chem. 1971 Sep 10;246(17):5365-72.
8
Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray crystallography.通过X射线晶体学研究鸟苷二磷酸与大肠杆菌延伸因子Tu结合的结构细节。
EMBO J. 1985 Sep;4(9):2385-8. doi: 10.1002/j.1460-2075.1985.tb03943.x.
9
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.蛋白激酶家族：催化结构域的保守特征与推导的系统发育
Science. 1988 Jul 1;241(4861):42-52. doi: 10.1126/science.3291115.
10
Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis.细菌趋化作用中的组氨酸磷酸化和磷酰基转移
Nature. 1988 Nov 10;336(6195):139-43. doi: 10.1038/336139a0.