Shibanaka Y, Hayashi H, Takai M, Fujita N
International Research Laboratories, Ciba-Geigy Ltd, Takarazuka, Japan.
Eur J Biochem. 1993 Feb 1;211(3):427-30. doi: 10.1111/j.1432-1033.1993.tb17567.x.
Eclosion hormone (EH), an insect neuropeotide, stimulated phosphatidylinositol (PtdIns) hydrolysis in abdominal ganglia isolated from Bombyx mori in a specific stage of adult development. Incubation of abdominal ganglia from silkworm pharate adults with EH led to an increase in formation of inositol 1,4,5-trisphosphate but this increase took place transiently, maximum increase being observed 30 s after the addition of EH. PtdIns hydrolysis was stimulated by exogenous EH in a dose-dependent fashion and was completely abolished by the phospholipase C inhibitors, neomycin and compound 48/80. The EH-induced PtdIns hydrolysis developed in parallel to the EH-induced eclosion behaviour during development of the adult. These results suggest that the EH-stimulated PtdIns hydrolysis plays an important role in EH-mediated signal transduction during adult development of B. mori.
羽化激素（EH）是一种昆虫神经肽，在家蚕成虫发育的特定阶段，它能刺激从家蚕分离出的腹神经节中的磷脂酰肌醇（PtdIns）水解。用EH孵育即将羽化的家蚕成虫的腹神经节，会导致肌醇1,4,5 -三磷酸的形成增加，但这种增加是短暂的，在添加EH后30秒观察到最大增加。外源性EH以剂量依赖的方式刺激PtdIns水解，并且被磷脂酶C抑制剂新霉素和化合物48/80完全消除。在成虫发育过程中，EH诱导的PtdIns水解与EH诱导的羽化行为平行发展。这些结果表明，EH刺激的PtdIns水解在家蚕成虫发育过程中EH介导的信号转导中起重要作用。
