上皮性阿米洛利敏感钠通道的表达克隆。一种与秀丽隐杆线虫退化蛋白具有同源性的新型通道。

Expression cloning of an epithelial amiloride-sensitive Na+ channel. A new channel type with homologies to Caenorhabditis elegans degenerins.

作者信息

Lingueglia E, Voilley N, Waldmann R, Lazdunski M, Barbry P

机构信息

Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.

出版信息

FEBS Lett. 1993 Feb 22;318(1):95-9. doi: 10.1016/0014-5793(93)81336-x.

DOI:10.1016/0014-5793(93)81336-x

PMID:8382172

Abstract

A complementary DNA encoding an amiloride-sensitive Na+ channel has been cloned and characterized from rat colon. The protein encoded by the cDNA has a sequence of 699 amino acids (79 kDa) containing several putative membrane spanning domains and potential phosphorylation sites. It forms a channel that has the electrophysiological and pharmacological properties characteristic of the epithelial Na+ channel. Homologies (including in transmembrane domains) have been found between a part of the channel sequence and the Mec4 gene product of Caenorhabditis elegans, a protein associated with mutation-induced neuronal degeneration.

摘要

一种编码阿米洛利敏感型钠离子通道的互补DNA已从大鼠结肠中克隆并鉴定。该cDNA编码的蛋白质有699个氨基酸序列（79 kDa），包含几个推定的跨膜结构域和潜在的磷酸化位点。它形成了一个具有上皮钠离子通道特征性电生理和药理特性的通道。在该通道序列的一部分与秀丽隐杆线虫的Mec4基因产物之间发现了同源性（包括跨膜结构域），Mec4基因产物是一种与突变诱导的神经元变性相关的蛋白质。