Fraefel C, Wirth U V, Vogt B, Schwyzer M
Institute of Virology, Faculty of Veterinary Medicine, University of Zürich, Switzerland.
J Virol. 1993 Mar;67(3):1328-33. doi: 10.1128/JVI.67.3.1328-1333.1993.
Herpesvirus genomes are linear molecules in virions. Prior to replication in host cells, they form circular templates by unknown mechanisms. Examining lytic infection with bovine herpesvirus 1, we observed immediate-early transcription over joined genome ends, which suggested that circles are present at the initial stage of infection. Among the transcripts was a spliced immediate-early RNA (1.5 kb) sharing exon 1 with previously described major immediate-early transcripts from the right genome end and exon 2 with a late transcript located near the left genome end. Exon 2 encodes a putative circ-encoded protein with homology to the varicella-zoster virus open reading frame 2 and equine herpesvirus 1 open reading frame 3 products. The novel features reported here for bovine herpesvirus 1 may constitute a more general property of herpesviruses.
疱疹病毒基因组在病毒粒子中是线性分子。在宿主细胞中复制之前，它们通过未知机制形成环状模板。在用牛疱疹病毒1进行裂解感染的研究中，我们观察到在连接的基因组末端存在立即早期转录，这表明在感染初期存在环状结构。在这些转录本中，有一种剪接的立即早期RNA（1.5 kb），其外显子1与先前描述的来自基因组右端的主要立即早期转录本相同，外显子2与位于基因组左端附近的一个晚期转录本相同。外显子2编码一种假定的环状编码蛋白，与水痘-带状疱疹病毒开放阅读框2和马疱疹病毒1开放阅读框3产物具有同源性。这里报道的牛疱疹病毒1的新特征可能构成疱疹病毒更普遍的特性。
