Laux G, Economou A, Farrell P J
Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, U.K.
J Gen Virol. 1989 Nov;70 ( Pt 11):3079-84. doi: 10.1099/0022-1317-70-11-3079.
The intact terminal protein genes (TP1 and TP2) of Epstein-Barr virus (EBV) are created upon infection by circularization of the linear viral genome at its terminal repeats. The structure of the 1.7 kb TP2 latent mRNA has been determined by cDNA analysis and Northern blotting, revealing its close relation to TP1 mRNA. The 1.7 kb transcript is expressed from a different promoter and has a different 5' exon from TP1 but is also spliced across the terminal repeats. The last eight exons are common to the TP1 and TP2 RNAs. The TP2 promoter is 3.3 kb downstream of the TP1 promoter and is part of a bidirectional latent EBV promoter region transcribing the TP2 and the latent membrane protein RNAs in opposite directions.
爱泼斯坦-巴尔病毒（EBV）完整的末端蛋白基因（TP1和TP2）是在感染时通过线性病毒基因组在其末端重复序列处环化而产生的。1.7 kb TP2潜伏性mRNA的结构已通过cDNA分析和Northern印迹法确定，显示出它与TP1 mRNA密切相关。1.7 kb转录本由不同的启动子表达，其5'外显子与TP1不同，但也跨越末端重复序列进行剪接。最后八个外显子是TP1和TP2 RNA共有的。TP2启动子位于TP1启动子下游3.3 kb处，是双向潜伏性EBV启动子区域的一部分，该区域以相反方向转录TP2和潜伏膜蛋白RNA。
