Sato M, Watanabe H, Ajioka Y, Noda Y, Sakai Y
First Department of Pathology, Niigata University School of Medicine, Japan.
Gastroenterol Jpn. 1993 Feb;28(1):72-80. doi: 10.1007/BF02775006.
Our objective was to compare the accuracy of differentiation of pancreatic carcinoma from the benign ductular aggregation of chronic pancreatitis using two methods, nuclear morphometric analysis and modified argyrophilic nucleolar organiser region (AgNOR) staining for nucleoli and dispersed NORs (AgNU staining). We evaluated 35 metastatic or invasive lesions of pancreatic carcinoma with low-grade atypia, 16 lesions of ductular aggregation and 12 lesions of proper epithelium. Four parameters, consisting of the size of nucleus: mean area, mean minor axis, mean major axis and mean perimeter, and two parameters of AgNu staining: mean number of AgNu-stained dots per nucleus (equal to mean number of nucleoli and dispersed NORs) and mean area of the largest AgNu-stained dot (equal to mean area of the largest nucleolus) of pancreatic carcinomas were significantly larger than those of ductular aggregations and of proper epithelia. In the discriminant analysis of four parameters of nuclear size, the discrimination of carcinomas was correct in 82.9% and that of ductular aggregations in 81.3%. Discriminant analysis by two parameters of AgNu staining showed 2 correct discrimination of lesions in 88.6% and 100%. Thus, AgNu staining was more accurate than nuclear morphometric analysis in discriminating a pancreatic carcinoma from ductular aggregation.
我们的目的是使用两种方法，即核形态计量分析和改良的嗜银核仁组成区（AgNOR）染色法对核仁和分散的NORs（AgNU染色），比较胰腺癌与慢性胰腺炎良性导管聚集的鉴别准确性。我们评估了35例低级别异型性的胰腺癌转移或浸润性病变、16例导管聚集病变和12例正常上皮病变。胰腺癌的四个参数，包括细胞核大小：平均面积、平均短轴、平均长轴和平均周长，以及AgNu染色的两个参数：每个细胞核AgNu染色点的平均数量（等于核仁和分散的NORs的平均数量）和最大AgNu染色点的平均面积（等于最大核仁的平均面积），均显著大于导管聚集和正常上皮的相应参数。在对细胞核大小四个参数的判别分析中，对癌的判别正确率为82.9%，对导管聚集的判别正确率为81.3%。通过AgNu染色的两个参数进行的判别分析显示，对病变的正确判别率分别为88.6%和100%。因此，在鉴别胰腺癌与导管聚集方面，AgNu染色比核形态计量分析更准确。
