pUC18的衍生物，其在修饰的多克隆位点两侧具有BglII位点，并保留了通过对大肠杆菌菌落进行视觉筛选来鉴定重组克隆的能力。

Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies.

作者信息

Janssen G R, Bibb M J

机构信息

Department of Biology, Indiana University, Bloomington 47405.

出版信息

Gene. 1993 Feb 14;124(1):133-4. doi: 10.1016/0378-1119(93)90774-w.

DOI:10.1016/0378-1119(93)90774-w

PMID:8382652

Abstract

We report the construction of a series of derivatives of pUC18 in which a modified multiple cloning site is flanked by one or two BglII sites. The reading frame of the lacZ alpha fragment was retained in each construct, permitting the recognition of transformants containing plasmids with inserts by visual screening for loss of blue coloration.

摘要

我们报道了一系列pUC18衍生物的构建，其中一个修饰的多克隆位点两侧是一个或两个BglII位点。在每个构建体中保留了lacZα片段的阅读框，通过目视筛选蓝色消失来识别含有插入质粒的转化体。