DeLaFuente A, Cisneros E, Talavera A
Centro Nacional de Biotecnología, Cantoblanco, Madrid, Spain.
Gene. 1994 Feb 11;139(1):83-6. doi: 10.1016/0378-1119(94)90527-4.
The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.
本文描述了四种具有新型多克隆位点（MCS）的载体的构建、表征及用途。这些多克隆位点由一个具有独特限制酶切位点的中心区域组成，两侧各有一个区域，其中四个位点以正向方式重复。这些载体允许插入具有相同或不同限制酶切末端的片段，并通过相同或不同末端的多种组合进行回收。该系统保留了pUC质粒的XGal蓝白筛选特性及其作为测序载体的用途。
