Phillips J D, Trumpower B L
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755.
Yeast. 1993 Jan;9(1):95-7. doi: 10.1002/yea.320090112.
We present here mapping data for QCR9, a nuclear gene encoding a subunit of the ubiquinol-cytochrome c oxidoreductase complex. Deletion of QCR9 results in the inability of cells to grow on grow on-fermentable carbon sources at 37 degrees C. Thus, qcr9 mutants can be scored by growing cells on YPE/G at 37 degrees C, or followed by the URA3 marker, which was inserted when making the qcr9 deletion strain, JDP1. The location of QCR9 on the right arm of chromosome VII with respect to the previously mapped genes ADE3, SER2 and PET54 is given.
我们在此展示了QCR9的定位数据,QCR9是一个编码泛醇 - 细胞色素c氧化还原酶复合体亚基的核基因。QCR9的缺失导致细胞在37摄氏度下无法在可发酵碳源上生长。因此,qcr9突变体可以通过在37摄氏度的YPE/G培养基上培养细胞来筛选,或者通过URA3标记来追踪,URA3标记是在构建qcr9缺失菌株JDP1时插入的。文中给出了QCR9在染色体VII右臂上相对于先前定位的基因ADE3、SER2和PET54的位置。