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用于构建重组病毒粒子的多功能腺相关病毒2型载体。

Versatile adeno-associated virus 2-based vectors for constructing recombinant virions.

作者信息

Nahreini P, Woody M J, Zhou S Z, Srivastava A

机构信息

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202-5120.

出版信息

Gene. 1993 Feb 28;124(2):257-62. doi: 10.1016/0378-1119(93)90402-o.

DOI:10.1016/0378-1119(93)90402-o
PMID:8383071
Abstract

We have constructed several plasmid vectors with which a more efficient molecular cloning, followed by rescue, replication, and packaging of DNA fragments, can be achieved. The availability of these vectors should facilitate construction of a variety of recombinant adeno-associated virus 2 (AAV)-based virions containing therapeutic genes for potential use in human gene therapy.

摘要

我们构建了几种质粒载体，利用这些载体可实现更高效的分子克隆，随后对DNA片段进行拯救、复制和包装。这些载体的可用性应有助于构建多种基于重组腺相关病毒2（AAV）的病毒粒子，这些病毒粒子含有可用于人类基因治疗的治疗性基因。

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