应激期间大鼠血浆提取物中促肾上腺皮质激素（ACTH）的皮质类固醇生成活性与免疫反应性ACTH比例的增加。

Increase of the proportion of corticosteroidogenic activity of ACTH versus immunoreactive ACTH in rat plasma extracts during stress.

作者信息

Goverde H J, Pesman G J, Smals A G

机构信息

Dept. of Endocrinology and Reproduction (III), Catholic University, Nijmegen, The Netherlands.

出版信息

Life Sci. 1993;52(11):959-64. doi: 10.1016/0024-3205(93)90531-7.

DOI:10.1016/0024-3205(93)90531-7

PMID:8383264

Abstract

The relationship between bioactive and immunoreactive adrenocorticotrophin (ACTH) was studied under various conditions. Rats were stressed by ether and the immunoreactive (I-ACTH) and bioactive (B-ACTH) ACTH levels were compared to the normal values obtained from "handled" rats. The bioactivity was determined by measuring the corticosteroid production in an isolated rat adrenal cell system. Stress exposure enhanced both the I-ACTH and B-ACTH levels. I-ACTH rose from 86.9 +/- 37.9 to 363 +/- 148.7 pg/ml, whereas the B-ACTH levels rose from 34.2 +/- 11.9 to 424.2 +/- 170.9 pg/ml. The bioactive to immunoreactive ratio (B/I ratio) was calculated as 0.47 +/- 0.13 in the control group and rose to the significantly higher value of 1.10 +/- 0.22 in the stressed rats. The conclusion is that under normal circumstances only about 50% of plasma immunoreactive ACTH shows corticosteroidogenic activity and that the B/I ACTH ratio might be a useful tool to estimate the physiological phase of the pituitary releasing activity.

摘要

在各种条件下研究了生物活性促肾上腺皮质激素（ACTH）与免疫反应性促肾上腺皮质激素之间的关系。用乙醚对大鼠进行应激处理，并将免疫反应性促肾上腺皮质激素（I-ACTH）和生物活性促肾上腺皮质激素（B-ACTH）水平与从“处理过”的大鼠获得的正常值进行比较。通过测量分离的大鼠肾上腺细胞系统中的皮质类固醇生成来确定生物活性。应激暴露增强了I-ACTH和B-ACTH水平。I-ACTH从86.9±37.9上升至363±148.7 pg/ml，而B-ACTH水平从34.2±11.9上升至424.2±170.9 pg/ml。对照组的生物活性与免疫反应性比值（B/I比值）计算为0.47±0.13，在应激大鼠中升至显著更高的值1.10±0.22。结论是，在正常情况下，血浆免疫反应性ACTH中只有约50%显示出皮质类固醇生成活性，并且B/I ACTH比值可能是估计垂体释放活性生理阶段的有用工具。