Kokawa N, Yamoto M, Furukawa K, Nakano R
Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
Acta Endocrinol (Copenh). 1993 Jan;128(1):88-94. doi: 10.1530/acta.0.1280088.
We performed partial purification of low molecular weight luteinizing hormone binding inhibitor from porcine follicular fluids and examined its biological activities. Following ultrafiltration, gel filtration and anion exchange of the pooled porcine follicular fluids, low molecular weight fractions (500-10,000 MW) inhibited [125I]hLH binding to porcine granulosa cells in a dose-dependent manner. The binding inhibition kinetics study revealed that the luteinizing hormone binding inhibitor may indicate a non-competitive inhibition with [125I]hLH binding. In vitro bioassay using adult mouse testicular interstitial cells revealed that the partially purified luteinizing hormone binding inhibitor reduced ovine LH-stimulated testosterone and cAMP production in a dose-dependent manner, whereas the luteinizing hormone binding inhibitor did not affect basal production of testosterone and cAMP. The inhibitory activity was heat stable and did not disappear with activated charcoal adsorption. The results of the present study suggest that the luteinizing hormone binding inhibitor may play an important role as an ovarian non-steroidal regulator modulating the receptor binding of LH and LH-mediated steroidogenesis.
我们从猪卵泡液中对低分子量促黄体生成素结合抑制剂进行了部分纯化，并检测了其生物学活性。对合并的猪卵泡液进行超滤、凝胶过滤和阴离子交换后，低分子量组分（500 - 10,000分子量）以剂量依赖方式抑制[125I]人促黄体生成素与猪颗粒细胞的结合。结合抑制动力学研究表明，促黄体生成素结合抑制剂可能对[125I]人促黄体生成素结合表现为非竞争性抑制。使用成年小鼠睾丸间质细胞进行的体外生物测定显示，部分纯化的促黄体生成素结合抑制剂以剂量依赖方式降低了绵羊促黄体生成素刺激的睾酮和环磷酸腺苷（cAMP）的产生，而促黄体生成素结合抑制剂并不影响睾酮和cAMP的基础产生。该抑制活性具有热稳定性，且不会因活性炭吸附而消失。本研究结果表明，促黄体生成素结合抑制剂可能作为一种卵巢非甾体调节剂，在调节促黄体生成素的受体结合和促黄体生成素介导的类固醇生成方面发挥重要作用。
