邓恩骨肉瘤细胞对二丁酰环磷腺苷的分化反应
Differentiation of Dunn osteosarcoma cells in response to dibutyryl cyclic 3',5'-adenosine monophosphate.
作者信息
Kanamori M, Matsui H, Yudoh K, Maeda A, Kadowaki K M, Tsuji H, Ochiai H, Tatezaki S
机构信息
Department of Orthopedic Surgery, Toyama Medical and Pharmaceutical University, Japan.
出版信息
J Cancer Res Clin Oncol. 1993;119(6):323-8. doi: 10.1007/BF01208839.
We investigated the effects of dibutyryl cyclic adenosine 3',5'-monophosphate (Bt2cAMP) on the differentiation of Dunn osteosarcoma cells. Flow-cytometric analysis and DNA synthesis assay showed that Bt2cAMP decreased the cell population in the S phase in a time- and dose-dependent manner. Also, the cells showed distinct morphological and functional alterations; the cell morphology changed to a fibroblast-like appearance with long and thin protoplasmic processes, the knobs or blebs on both the cell membrane and nuclear membrane disappeared and the intracellular alkaline phosphatase activity increased. Moreover, Bt2cAMP-treated cells secreted a large quantity of fibronectin, which was deposited on the extended cell surface in the culture medium. Thus, Dunn osteosarcoma cells are differentiated morphologically and functionally by Bt2cAMP, and might be transformed to benign precursor cells.
我们研究了二丁酰环磷腺苷（Bt2cAMP）对邓恩骨肉瘤细胞分化的影响。流式细胞术分析和DNA合成测定表明，Bt2cAMP以时间和剂量依赖性方式减少了S期的细胞数量。此外，细胞表现出明显的形态和功能改变；细胞形态变为具有细长原生质突起的成纤维细胞样外观，细胞膜和核膜上的瘤或泡消失，细胞内碱性磷酸酶活性增加。此外，经Bt2cAMP处理的细胞分泌大量纤连蛋白，其沉积在培养基中伸展的细胞表面上。因此，邓恩骨肉瘤细胞在形态和功能上通过Bt2cAMP分化，并且可能转化为良性前体细胞。
Zotero 插件