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灵长类动物中央凹的遮阳伞(Pα)神经节细胞:用抗GABAA受体抗体进行免疫细胞化学染色。

Parasol (P alpha) ganglion-cells of the primate fovea: immunocytochemical staining with antibodies against GABAA-receptors.

作者信息

Grünert U, Greferath U, Boycott B B, Wässle H

机构信息

Max-Planck-Institut für Hirnforschung, Frankfurt/Main, Germany.

出版信息

Vision Res. 1993 Jan;33(1):1-14. doi: 10.1016/0042-6989(93)90052-x.

DOI:10.1016/0042-6989(93)90052-x
PMID:8383899
Abstract

Retinae of macaque monkeys were immuno-stained with antibodies against GABAA-receptors. In peripheral retina most ganglion cells were immunoreactive. In central retina, around the fovea, staining in the ganglion cell layer was selective and only 5-8% of all ganglion cells were labelled: these had the largest cell bodies and their dendrites occupied a broad stratum in the middle of the inner plexiform layer. From comparison with Golgi-stained ganglion cells it is concluded that the entire population of parasol (P alpha)-cells at the fovea was labelled. The mosaic and sampling properties of parasol cells were determined by combining dendritic field measurements of Golgi-stained cells with their density when immuno-stained. There is convergence of 30-50 cones onto each foveal parasol ganglion cell. The dendritic fields of both ON- and OFF-parasol cells provide complete retinal coverage. The Nyquist limits of their mosaics are 4 min of arc.

摘要

用抗γ-氨基丁酸A型受体(GABAA-receptors)抗体对猕猴视网膜进行免疫染色。在周边视网膜,大多数神经节细胞具有免疫反应性。在中央视网膜,在中央凹周围,神经节细胞层的染色具有选择性,所有神经节细胞中只有5 - 8%被标记:这些细胞具有最大的细胞体,其树突占据内网状层中部的一个宽层。通过与高尔基染色的神经节细胞比较得出结论,中央凹处的整个伞状(Pα)细胞群体都被标记。通过将高尔基染色细胞的树突野测量与其免疫染色时的密度相结合,确定了伞状细胞的镶嵌和采样特性。每个中央凹伞状神经节细胞上有30 - 50个视锥细胞汇聚。ON-和OFF-伞状细胞的树突野提供了完整的视网膜覆盖。它们镶嵌的奈奎斯特极限为4分视角。

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