Uchida T, Nakagawaji K, Kojima H, Tokumaru T, Imaizumi M, Abe T
Dept. of Surgery, Aichi Prefectural Hospital.
Gan To Kagaku Ryoho. 1993 Mar;20(4):467-72.
Tumor cells derived from a metastatic mediastinal lymph node of a thirty six year old man with large cell lung cancer were transplanted into the subcapsule of the kidney of a nude mouse. The growing tumor was transplanted into a nude mouse twice and, thereafter, cultured in vitro. After the twelfth passage in vitro, the cell (NUTIY) was transplanted into subcutaneous tissue of a nude mouse and formed a rapidly growing tumor, showing the histological pattern similar to the large cell lung cancer of the patient. However, the chromosomal analysis revealed only murine chromosomes for NUTIY and any transfected human genes were not found in the genes of NUTIY by means of Alu probe. H-ras oncogene of NUTIY was amplified 4 folds, but that of the primary lung cancer and the metastatic lymph node of the patient was on a level with normal cells. NUTIY might be a cancer cell which a murine cell had been transformed into by some carcinogen in vitro.
从一名36岁大细胞肺癌男性患者的转移性纵隔淋巴结中获取的肿瘤细胞，被移植到一只裸鼠肾脏的被膜下。生长的肿瘤被移植到裸鼠体内两次，之后进行体外培养。在体外传代12次后，将该细胞（NUTIY）移植到裸鼠的皮下组织，形成了快速生长的肿瘤，其组织学模式与患者的大细胞肺癌相似。然而，染色体分析显示NUTIY仅含有小鼠染色体，通过Alu探针在NUTIY的基因中未发现任何转染的人类基因。NUTIY的H-ras癌基因扩增了4倍，但患者原发性肺癌和转移性淋巴结的H-ras癌基因水平与正常细胞相当。NUTIY可能是一种在体外被某种致癌物转化成的鼠类癌细胞。
