Gravestein L A, Blom B, Nolten L A, de Vries E, van der Horst G, Ossendorp F, Borst J, Loenen W A
Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Eur J Immunol. 1993 Apr;23(4):943-50. doi: 10.1002/eji.1830230427.
CD27 is a member of the nerve growth factor receptor family, that includes two types of tumor necrosis factor receptor, CD40 and Fas/Apo-1. Human CD27 has been found only on lymphocytes. In T cells, its expression strongly increases in a transient fashion upon antigenic stimulation, suggesting that CD27 plays a role during T cell activation. To analyze the function of CD27, we have identified the murine CD27 at the cDNA and protein level. Murine CD27 shows an identity of 65% compared with human CD27. The amino-terminal cysteine-rich region, i.e. the putative ligand-binding domain, and the carboxy-terminal part of the cytoplasmic domain are approximately 80% identical in man and mouse. Murine CD27 has 29% identity to 4-1BB, another lymphocyte-specific member of the receptor family defined only at the cDNA level. Murine CD27 and 4-1BB have 39% homology in the cysteine-rich domain and share a conserved region in the cytoplasmic tail. Expression studies identified murine CD27 mRNA in thymus and spleen, but not in non-lymphoid tissues, while 4-1BB mRNA was not detected in any tissue tested. In resting T cells, only murine CD27 mRNA was found, while in activated T cells murine CD27 as well as 4-1BB were present at high levels. Murine CD27 and 4-1BB mRNA are expressed with different kinetics during T cell activation, suggesting that these molecules play different roles in this process. Peptide antisera identified murine CD27 as a 45-kDa protein on thymocytes and activated T cells, while 4-1BB was precipitated as a 35-40-kDa protein from activated T cells.
CD27是神经生长因子受体家族的一员，该家族包括两种肿瘤坏死因子受体，即CD40和Fas/Apo-1。人类CD27仅在淋巴细胞上被发现。在T细胞中，其表达在抗原刺激后以短暂的方式强烈增加，这表明CD27在T细胞激活过程中发挥作用。为了分析CD27的功能，我们在cDNA和蛋白质水平上鉴定了小鼠CD27。与人类CD27相比，小鼠CD27的同源性为65%。人鼠氨基末端富含半胱氨酸的区域，即假定的配体结合结构域，以及细胞质结构域的羧基末端部分大约80%相同。小鼠CD27与4-1BB有29%的同源性，4-1BB是仅在cDNA水平上定义的受体家族的另一个淋巴细胞特异性成员。小鼠CD27和4-1BB在富含半胱氨酸的结构域中有39%的同源性，并且在细胞质尾巴中有一个保守区域。表达研究在胸腺和脾脏中鉴定出小鼠CD27 mRNA，但在非淋巴组织中未检测到，而在任何测试组织中均未检测到4-1BB mRNA。在静止T细胞中，仅发现小鼠CD27 mRNA，而在活化T细胞中，小鼠CD27以及4-1BB均高水平存在。小鼠CD27和4-1BB mRNA在T细胞激活过程中以不同的动力学表达，这表明这些分子在该过程中发挥不同的作用。肽抗血清在胸腺细胞和活化T细胞上鉴定出小鼠CD27为一种45 kDa的蛋白质，而从活化T细胞中沉淀出的4-1BB为一种35 - 40 kDa的蛋白质。
