Liu Ting, Ma Yifei, Wang Zhiling, Zhang Wenjing, Yang Xingsheng
Department of Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People's Republic of China.
Department of Obstetrics and Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People's Republic of China.
Cancer Manag Res. 2020 Jan 15;12:303-311. doi: 10.2147/CMAR.S232994. eCollection 2020.
Cervical cancer is the second most common female malignancies. But the exact etiology of cervical cancer is still under investigation. Recent observations revealed that the loss expression of Siva 1 was related to several different types of tumors. It could play an indispensable role in both exogenous and endogenous apoptotic signaling pathways. Nevertheless, the relationship between Siva 1 expression and cervical cancer progression has not yet been fully clarified. This study aimed to explore the functional role of Siva1 in cervical cancer.
In this present experiment, expression of Siva 1 was detected in 87 cervical cancer, 34 CIN and 20 normal samples by immunohistochemistry. The correlation of Siva 1 expression and overall survival times (OS) was analyzed by Kaplan-Meier analysis. We up-regulated the expression of Siva 1 by plasmid pCMV3-Siva 1 in C33A cells. CCK8, flow cytometry, wound-healing, and transwell assays were performed to examine the influences of Siva 1 expression on cell proliferation, apoptosis, migration and invasion.
The expression of Siva 1 was decreased in cervical cancer tissues compared with CIN and normal tissues. In addition, the Siva 1 immunoreactivity was significantly associated with tumor differentiation. Patients with Siva 1 negative staining exhibited a significantly decreased overall survival. Then, we established stable Siva 1 ectopic expression cells, and we found that elevated expression of Siva 1 promoted apoptosis, inhibited proliferation, and suppressed migration and invasion of cervical cancer cells.
The present study revealed a crucial role of Siva 1 in tumor progression and it may be a valuable prognostic indicator of cervical cancer.
宫颈癌是第二常见的女性恶性肿瘤。但其确切病因仍在研究中。最近的观察表明，Siva 1的表达缺失与几种不同类型的肿瘤有关。它在外源性和内源性凋亡信号通路中都可能发挥不可或缺的作用。然而，Siva 1表达与宫颈癌进展之间的关系尚未完全阐明。本研究旨在探讨Siva1在宫颈癌中的功能作用。
在本实验中，通过免疫组织化学检测了87例宫颈癌、34例宫颈上皮内瘤变（CIN）和20例正常样本中Siva 1的表达。采用Kaplan-Meier分析方法分析Siva 1表达与总生存时间（OS）的相关性。我们通过质粒pCMV3-Siva 1在C33A细胞中上调Siva 1的表达。进行CCK8、流式细胞术、伤口愈合和Transwell实验，以检测Siva 1表达对细胞增殖、凋亡、迁移和侵袭的影响。
与CIN和正常组织相比，宫颈癌组织中Siva 1的表达降低。此外，Siva 1免疫反应性与肿瘤分化显著相关。Siva 1阴性染色的患者总生存率显著降低。然后，我们建立了稳定的Siva 1异位表达细胞，发现Siva 1表达升高可促进宫颈癌细胞凋亡、抑制增殖并抑制其迁移和侵袭。
本研究揭示了Siva 1在肿瘤进展中的关键作用，它可能是宫颈癌一个有价值的预后指标。
