Aducci P, Ballio A, Donini V, Fogliano V, Fullone M R, Marra M
Department of Biology, II University of Rome Tor Vergata, Italy.
FEBS Lett. 1993 Apr 5;320(2):173-6. doi: 10.1016/0014-5793(93)80086-a.
Biochemical properties of fusicoccin receptors are strongly influenced by the phospholipid environment. In this report we have studied the effect of different exogenous phospholipases on fusicoccin binding ability of both plasma membrane and solubilised receptors. Among the phospholipases tested only phospholipase A2 showed an inhibitory effect on fusicoccin binding. In particular, the influence of this enzyme on the time course and reversibility of the fusicoccin binding reaction was studied. The inhibitory effect of phospholipase A2 was the consequence of fatty acid release. The usual fatty acids of plasma membrane phospholipids were active in inhibiting the interaction of fusicoccin with its receptors. It is concluded that a phospholipid associated to the fusicoccin receptor might play a significant role in the modulation of binding.
壳梭孢菌素受体的生化特性受磷脂环境的强烈影响。在本报告中，我们研究了不同外源性磷脂酶对质膜和可溶性受体的壳梭孢菌素结合能力的影响。在所测试的磷脂酶中，只有磷脂酶A2对壳梭孢菌素结合表现出抑制作用。特别地，研究了该酶对壳梭孢菌素结合反应的时间进程和可逆性的影响。磷脂酶A2的抑制作用是脂肪酸释放的结果。质膜磷脂的常见脂肪酸在抑制壳梭孢菌素与其受体的相互作用方面具有活性。得出的结论是，与壳梭孢菌素受体相关的磷脂可能在结合调节中起重要作用。
