• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Rapid and convenient purification of tonin from rat submandibular gland. Comparison of tonin and tissue kallikrein in their interactions with inhibitors.

作者信息

el-Thaher T, Bailey G S

机构信息

Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.

出版信息

Int J Pept Protein Res. 1993 Feb;41(2):196-200. doi: 10.1111/j.1399-3011.1993.tb00131.x.

DOI:10.1111/j.1399-3011.1993.tb00131.x
PMID:8384607
Abstract

Tonin was isolated from rat submandibular glands by a very convenient procedure consisting of sequential anion-exchange, hydrophobic interaction and gel filtration chromatographies. The method is superior to earlier purifications as it consists of fewer stages, resulting in a much higher recovery (41%) of tonin. The final preparation was seen to be pure on SDS gel electrophoresis (M(r) 32,800) and on gel isoelectric focusing (pI 6.15). The stability of tonin and its interaction with various inhibitors were investigated, and compared with the corresponding behavior of rat tissue kallikrein.

摘要

相似文献

1
Rapid and convenient purification of tonin from rat submandibular gland. Comparison of tonin and tissue kallikrein in their interactions with inhibitors.
Int J Pept Protein Res. 1993 Feb;41(2):196-200. doi: 10.1111/j.1399-3011.1993.tb00131.x.
2
Release of kallikrein and tonin from the rat submandibular gland.
Adv Exp Med Biol. 1986;198 Pt A:247-54. doi: 10.1007/978-1-4684-5143-6_34.
3
Rapid purification of tonin, esterase B, antigen psi and kallikrein from rat submandibular gland by fast protein liquid chromatography.
J Chromatogr. 1987 Jan 30;387:347-59. doi: 10.1016/s0021-9673(01)94537-6.
4
Isolation, characterization, and localization of antigen gamma, a serine proteinase of the "kallikrein-family" in the rat submandibular gland.
Biol Chem Hoppe Seyler. 1987 Nov;368(11):1455-67. doi: 10.1515/bchm3.1987.368.2.1455.
5
New specific assays for tonin and tissue kallikrein activities in rat submandibular glands. Assays reveal differences in the effects of sympathetic and parasympathetic stimulation on proteinases in saliva.
Biochem Pharmacol. 1992 Mar 17;43(6):1209-17. doi: 10.1016/0006-2952(92)90494-4.
6
Tonin, an esteroprotease from rat submaxillary glands.托宁，一种来自大鼠颌下腺的酯蛋白酶。
Biochim Biophys Acta. 1981 Jul 24;660(1):23-9. doi: 10.1016/0005-2744(81)90103-0.
7
Purification of tonin by affinity chromatography.
Hypertension. 1981 Jan-Feb;3(1):81-6. doi: 10.1161/01.hyp.3.1.81.
8
Tonin and kallikrein-kinin system.
Adv Exp Med Biol. 1989;247A:573-80. doi: 10.1007/978-1-4615-9543-4_89.
9
Immunohistochemical localization of tonin and its relation to kallikrein in rat salivary glands.
J Histochem Cytochem. 1982 Nov;30(11):1123-9. doi: 10.1177/30.11.6292285.
10
Role of calcium and calmodulin in release of kallikrein and tonin from rat submandibular gland.钙和钙调蛋白在大鼠下颌下腺激肽释放酶和血管紧张素释放中的作用。
Am J Physiol. 1986 Mar;250(3 Pt 1):C480-5. doi: 10.1152/ajpcell.1986.250.3.C480.