克隆大鼠甲状腺细胞系中的促甲状腺激素依赖性钾通道。

The TSH-dependent potassium channel in a cloned rat thyroid cell line.

作者信息

Yoshida A, Hisatome I, Kotake H, Taniguchi S, Sato R, Kouchi T, Ueta Y, Mitani Y, Shigemasa C, Mashiba H

机构信息

First Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.

出版信息

Biochem Biophys Res Commun. 1993 Mar 15;191(2):595-600. doi: 10.1006/bbrc.1993.1259.

DOI:10.1006/bbrc.1993.1259

PMID:8384846

Abstract

We found a TSH-dependent K+ channel in the membrane of a rat thyroid cell line (FRTL-5 cell). This K+ channel is activated by extracellular application of the thyroid stimulating hormone (TSH). Treatment of dibutyryladenosine cyclic monophosphate (Bt2 cAMP) also activated the TSH-dependent K+ channel. Intracellular application of protein kinase A activates this K+ channel without the presence of free Ca2+. These findings indicate that the thyroid K+ channel is activated through the TSH-cAMP-protein kinase A system. This is the first report of a polypeptide hormone activated K+ channel and it should be useful for examining the effects of this K+ channel on thyroid cell functions.

摘要

我们在大鼠甲状腺细胞系（FRTL-5细胞）的细胞膜中发现了一种促甲状腺激素（TSH）依赖性钾通道。这种钾通道可通过在细胞外施加促甲状腺激素（TSH）而被激活。用二丁酰腺苷环化一磷酸（Bt2 cAMP）处理也可激活TSH依赖性钾通道。在不存在游离Ca2+的情况下，向细胞内施加蛋白激酶A可激活此钾通道。这些发现表明，甲状腺钾通道是通过TSH-cAMP-蛋白激酶A系统被激活的。这是关于多肽激素激活钾通道的首次报道，它对于研究这种钾通道对甲状腺细胞功能的影响应该是有用的。