Graham G J, Zhou L, Weatherbee J A, Tsang M L, Napolitano M, Leonard W J, Pragnell I B
Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Bearsden, Glasgow, United Kingdom.
Cell Growth Differ. 1993 Mar;4(3):137-46.
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent stem cell inhibitor and a member of a large and expanding family of related cytokines. In an effort to understand the molecular basis of the activities of MIP-1 alpha, we have sought to characterize the cellular receptors for this molecule. Our results demonstrate the presence of abundant MIP-1 alpha receptors on both human and murine cells. The receptor on K562 cells can bind a range of members of the MIP-1 alpha family and may thus be a general MIP-1 alpha family receptor. Murine FDCPmix cells also bind a range of members of this peptide family, although the receptor(s) that they express appear somewhat more selective for peptides capable of displaying stem cell inhibitory properties. The human and murine receptors do not bind members of the related interleukin 8 family of peptides and are thus distinct from the recently cloned interleukin 8 receptor. We suggest that the receptor on the murine cell is a candidate for the receptor responsible for articulating stem cell inhibitory signals following MIP-1 alpha binding.
巨噬细胞炎性蛋白1α（MIP-1α）是一种有效的干细胞抑制剂，属于一个庞大且不断扩大的相关细胞因子家族。为了理解MIP-1α活性的分子基础，我们试图对该分子的细胞受体进行表征。我们的结果表明，人和鼠细胞上均存在丰富的MIP-1α受体。K562细胞上的受体可以结合MIP-1α家族的一系列成员，因此可能是一种通用的MIP-1α家族受体。鼠FDCPmix细胞也能结合该肽家族的一系列成员，尽管它们表达的受体对能够表现出干细胞抑制特性的肽似乎更具选择性。人和鼠的受体不结合相关的白细胞介素8肽家族成员，因此与最近克隆的白细胞介素8受体不同。我们认为，鼠细胞上的受体是负责传递MIP-1α结合后干细胞抑制信号的受体的候选者。
