Siddiqui Y M, al-Ahdal M N
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.
New Microbiol. 1993 Jan;16(1):95-8.
Plaque assay was performed on herpes simplex type 1, using Vero, BHK-21, Hep-2 and LLCMK2 cell lines. The plaquing efficiency was optimal when 60 mm dishes containing Vero cells received an inoculum of 200 microliters with an adsorption time of 90 min. The overlay contained agar and tissue culture medium supplemented with extra vitamins, DEAE-Dextran and magnesium chloride. Virus titer was increased by two fold and plaques were clearly visible within three days of incubation. This modified method was reproducible and is being used routinely in our research and diagnostic laboratories.
采用Vero、BHK - 21、Hep - 2和LLCMK2细胞系对1型单纯疱疹病毒进行空斑试验。当含有Vero细胞的60 mm培养皿接种200微升接种物，吸附时间为90分钟时，空斑形成效率最佳。覆盖物包含琼脂和补充了额外维生素、二乙氨基乙基葡聚糖和氯化镁的组织培养基。病毒滴度提高了两倍，在培养三天内空斑清晰可见。这种改良方法具有可重复性，并且正在我们的研究和诊断实验室中常规使用。
