Isaac J T, Wheal H V
Department of Physiology and Pharmacology, University of Southampton, UK.
Neurosci Lett. 1993 Feb 19;150(2):227-30. doi: 10.1016/0304-3940(93)90542-s.
Whole-cell voltage-clamped currents were recorded using low resistance patch electrodes containing K+ channel blockers with the local anaesthetic QX-314 (20mM). Under these conditions voltage-gated Na+ conductances and regenerative spiking activity were blocked, leaving Ca2+ conductances. The effect of QX-314 resembled that of extracellular tetrodotoxin but had the advantage of being localised to one cell therefore permitting recording of synaptic currents. Excitatory synaptic current recordings were progressively improved over the first 25 minutes of recording as input resistance increased. After this time postsynaptic currents were stable for at least 1 h.
使用含有钾通道阻滞剂和局部麻醉剂QX-314（20 mM）的低电阻膜片电极记录全细胞电压钳制电流。在这些条件下，电压门控钠电导和再生性动作电位发放活动被阻断，仅留下钙电导。QX-314的作用类似于细胞外河豚毒素，但具有局限于单个细胞的优势，因此允许记录突触电流。随着记录开始后的前25分钟内输入电阻增加，兴奋性突触电流记录逐渐改善。在此之后，突触后电流至少1小时保持稳定。
