Rubin B, Gouaillard C, Wiederanders G, Kuhlmann J
Laboratoire d'Immunologie cellulaire et moléculaire, CRPG/CNRS, Toulouse, France.
Scand J Immunol. 1993 Apr;37(4):479-86. doi: 10.1111/j.1365-3083.1993.tb03322.x.
It has been demonstrated that induction of immune responses, infectious diseases and autoimmune manifestations can be associated with at least four gene loci: the major histocompatibility complex (MHC) locus; the immunoglobulin (Ig) heavy chain (Hc) locus; and the T-cell receptor (TCR) TCR-alpha or TCR-beta chain loci. In the present study, we have analysed whether T-cell responses of IE-negative C57Bl/6 (B6) mice to IE alloantigen (IE alpha transgenic B6 mice = B6.E alpha 16) or to trinitrophenylated (TNP) syngeneic spleen cells were influenced by changes in the Ig-Hc locus or the TCRa locus. Whereas the fine specificity of T-cell responses to IE alloantigen was the same in B6 mice and in Ig-Hc congenic B6.26a or TCRa congenic B6.10TCa mice, the latter strain of mice demonstrated much higher IE-specific T-cell responses against B6.E alpha 16 spleen cells than B6 or B6.26a mice. This high responsiveness was a dominant feature and associated with the TCRa locus. In addition, the TCRV alpha or V beta repertoire of the congenic strains of mice was polyclonal and very similar. The TNP-specific T-cell responses of B6 and B6.10TCa mice showed the same restricted TCRV alpha and V beta repertoire. It is concluded that in both an oligoclonal T-cell response (anti-TNP) and a polyclonal T-cell response (anti-IE), exchange of Ig-Hc or TCRa loci does not significantly influence the TCRV alpha or V beta repertoire in IE-negative C57Bl/6 mice.
业已证明，免疫应答、感染性疾病及自身免疫表现的诱导至少与四个基因位点相关：主要组织相容性复合体（MHC）位点；免疫球蛋白（Ig）重链（Hc）位点；以及T细胞受体（TCR）的TCR-α或TCR-β链位点。在本研究中，我们分析了IE阴性的C57Bl/6（B6）小鼠对IE同种异体抗原（IEα转基因B6小鼠 = B6.Eα16）或三硝基苯化（TNP）同基因脾细胞的T细胞应答是否受Ig-Hc位点或TCRα位点变化的影响。虽然B6小鼠以及Ig-Hc同基因的B6.26a或TCRα同基因的B6.10TCa小鼠对IE同种异体抗原的T细胞应答精细特异性相同，但后一种品系的小鼠对B6.Eα16脾细胞表现出比B6或B6.26a小鼠更高的IE特异性T细胞应答。这种高反应性是一个显性特征，且与TCRα位点相关。此外，同基因品系小鼠的TCRVα或Vβ库是多克隆的且非常相似。B6和B6.10TCa小鼠的TNP特异性T细胞应答显示出相同的受限TCRVα和Vβ库。得出的结论是，在寡克隆T细胞应答（抗-TNP）和多克隆T细胞应答（抗-IE）中，Ig-Hc或TCRα位点的交换不会显著影响IE阴性的C57Bl/6小鼠中的TCRVα或Vβ库。
