Zahler A M, Neugebauer K M, Lane W S, Roth M B
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Science. 1993 Apr 9;260(5105):219-22. doi: 10.1126/science.8385799.
Alternative splicing of precursor messenger RNAs (pre-mRNAs) is a common mechanism of regulating gene expression. SR proteins are a family of pre-mRNA splicing factors that are structurally related and evolutionarily conserved. Any member of the SR family can complement a splicing-deficient extract that lacks the entire family of SR proteins. Here it is demonstrated that particular SR proteins have distinct functions in alternative pre-mRNA splicing in vitro. In addition, SR proteins are differentially expressed in a variety of tissues. These results suggest a fundamental role for SR proteins in the regulation of alternative splicing.
前体信使核糖核酸（pre-mRNA）的可变剪接是调节基因表达的一种常见机制。SR蛋白是一类前体mRNA剪接因子，它们在结构上相关且在进化上保守。SR家族的任何成员都可以补充缺乏整个SR蛋白家族的剪接缺陷提取物。本文证明了特定的SR蛋白在体外前体mRNA可变剪接中具有不同的功能。此外，SR蛋白在多种组织中差异表达。这些结果表明SR蛋白在可变剪接调控中具有重要作用。
