拟南芥中一个可移动内源性转座子的鉴定。
Identification of a mobile endogenous transposon in Arabidopsis thaliana.
作者信息
Tsay Y F, Frank M J, Page T, Dean C, Crawford N M
机构信息
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
出版信息
Science. 1993 Apr 16;260(5106):342-4. doi: 10.1126/science.8385803.
A mobile endogenous transposable element, Tag1, has been identified in the plant Arabidopsis thaliana. Tag1 was found in the nitrate transporter gene, CHL1, of a chlorate-resistant mutant present in a population of plants containing an active maize Ac transposon. Tag1 excises from the chl1 gene producing chlorate-sensitive revertants with Tag1 or Tag1-related elements at different loci. Tag1 and related elements are present in the Landsberg but not Columbia or Wassilewskija ecotypes of Arabidopsis. Thus, Tag1 provides a tool for the insertional mutagenesis of plant genes essential for biological processes of agronomic importance.
在植物拟南芥中发现了一种可移动的内源性转座元件Tag1。Tag1存在于一个含有活跃玉米Ac转座子的植物群体中一个抗氯酸盐突变体的硝酸盐转运蛋白基因CHL1中。Tag1从chl1基因中切除，产生对氯酸盐敏感的回复突变体，在不同位点带有Tag1或与Tag1相关的元件。Tag1及相关元件存在于拟南芥的Landsberg生态型中，但不存在于Columbia或Wassilewskija生态型中。因此，Tag1为对农艺重要生物学过程所必需的植物基因进行插入诱变提供了一种工具。
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