Cullen J M, Sandgren E P, Brinster R L, Maronpot R R
Department of Microbiology, Pathology, and Parasitology, North Carolina State University, College of Veterinary Medicine, Raleigh.
Vet Pathol. 1993 Mar;30(2):111-8. doi: 10.1177/030098589303000203.
The development of hepatic neoplasms was histologically characterized in transgenic mice that expressed an albumin enhancer-promoter/SV40 T-antigen fusion gene. At least five transgenic and three control mice were examined at monthly intervals over a 3-month period. At 1 month of age, five transgenic mice (two male, three female) and three controls (one male, two female) were examined. Five transgenic mice (two male, three female) and three controls (one male, two female) were examined at 2 months of age. Fourteen transgenic mice (12 male, two female) and three controls (two male, one female) were examined at 3 months of age. At 1 month of age, liver-to-body weight ratios of transgenic mice were increased nearly twofold as compared with controls. Histologically, livers from transgenic mice were characterized by dysplastic hepatocytes with marked variation in nucleus and cell size. At 2 months of age, livers from transgenic mice were 2.5 times larger than control livers and contained numerous 1-5-mm cystic spaces. Transgenic livers also contained multiple eosinophilic, basophilic, and clear foci, as well as cystic, hyperplastic bile ducts and biliary adenomas. At 3 months of age, transgenic livers were enlarged over eightfold as compared with controls and contained numerous cysts and solid masses up to 2 cm in diameter. Trabecular, glandular, and anaplastic hepatocellular carcinomas, as well as benign and malignant biliary neoplasms, were diagnosed. No metastasis was observed. Subcutaneous trabecular hepatocellular carcinomas developed in two of three syngeneic mice that had received transplants of a solid hepatic neoplasm, confirming the neoplastic behavior of these tumors.(ABSTRACT TRUNCATED AT 250 WORDS)
在表达白蛋白增强子 - 启动子/SV40 T抗原融合基因的转基因小鼠中，对肝脏肿瘤的发展进行了组织学特征研究。在3个月的时间里，每月对至少5只转基因小鼠和3只对照小鼠进行检查。1月龄时，检查了5只转基因小鼠（2只雄性，3只雌性）和3只对照小鼠（1只雄性，2只雌性）。2月龄时，检查了5只转基因小鼠（2只雄性，3只雌性）和3只对照小鼠（1只雄性，2只雌性）。3月龄时，检查了14只转基因小鼠（12只雄性，2只雌性）和3只对照小鼠（2只雄性，1只雌性）。1月龄时，转基因小鼠的肝体重比与对照相比增加了近两倍。组织学上，转基因小鼠的肝脏特征为发育异常的肝细胞，细胞核和细胞大小有明显差异。2月龄时，转基因小鼠的肝脏比对照肝脏大2.5倍，含有许多1 - 5毫米的囊性间隙。转基因肝脏还含有多个嗜酸性、嗜碱性和透明病灶，以及囊性、增生性胆管和胆管腺瘤。3月龄时，转基因肝脏比对照肝脏增大了八倍多，含有许多囊肿和直径达2厘米的实性肿块。诊断出小梁状、腺管状和间变性肝细胞癌，以及良性和恶性胆管肿瘤。未观察到转移。在接受实体肝肿瘤移植的三只同基因小鼠中的两只中发生了皮下小梁状肝细胞癌，证实了这些肿瘤的肿瘤行为。（摘要截断于250字）
