Lipkin V M, Bondarenko V A, Zagranichny V E, Dobrynina L N, Muradov K G
Branch of Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region.
Biochim Biophys Acta. 1993 Apr 16;1176(3):250-6. doi: 10.1016/0167-4889(93)90052-q.
The recombinant and 21 mutant phosphodiesterase (PDE) gamma subunit (PDE gamma) genes were expressed by sequential transcription and translation in vitro. Inhibitory properties of these mutants and their interactions with PDE catalytic and transducin alpha subunits were studied. The interaction of the PDE gamma subunit with the catalytic ones proceeds in two steps--primary binding and inhibition. The central region of the PDE gamma molecule enriched with the basic amino acid residues (particularly, Lys-29, Lys-31 and Arg-33), is involved in the primary binding, and the PDE gamma C-terminus plays the key role in inhibition. The spatial orientation of the C-terminus is of great importance here. The PDE gamma C-terminus also affects binding to catalytic moieties.
通过体外顺序转录和翻译表达了重组的和21种突变型磷酸二酯酶（PDE）γ亚基（PDEγ）基因。研究了这些突变体的抑制特性及其与PDE催化亚基和转导蛋白α亚基的相互作用。PDEγ亚基与催化亚基的相互作用分两步进行——初次结合和抑制。富含碱性氨基酸残基（特别是赖氨酸-29、赖氨酸-31和精氨酸-33）的PDEγ分子中心区域参与初次结合，而PDEγ的C末端在抑制中起关键作用。在此，C末端的空间取向非常重要。PDEγ的C末端也影响与催化部分的结合。
