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[环氧树脂包埋块半薄切片在视网膜细胞学分析中的应用]

[Use of semithin sections from epoxy blocks in the cytological analysis of the retina].

作者信息

Manokhina M S, Bakhtybaev O E

出版信息

Arkh Patol. 1978;40(11):66-9.

PMID:83860
Abstract

The cytological features of the cell structure of various layers of the retina are revealed better and with greater detail when semi-thin sections from epoxy blocks are stained with basophilic stains (toluidine blue, methylene blue, thionine) than when conventional histological sections are stained with the same stains. Semi-thin sections are suitable for light microscope cytological analysis of relatively large segments of the retina (3 X 8 mm). In future, this material will probably be used for electron microscopic examinations. The currently available methods for staining of semi-thin sections with basophilic stains are relatively simple and produce stable results.

摘要

当用嗜碱性染料(甲苯胺蓝、亚甲蓝、硫堇)对环氧树脂包埋块的半薄切片进行染色时,视网膜各层细胞结构的细胞学特征比用相同染料对传统组织学切片进行染色时显示得更好、更详细。半薄切片适用于对相对较大的视网膜区域(3×8毫米)进行光学显微镜细胞学分析。将来,这种材料可能会用于电子显微镜检查。目前可用的用嗜碱性染料对半薄切片进行染色的方法相对简单,且结果稳定。

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