Stewart E M, Stevenson M H, Gray R
Food and Agricultural Chemistry Research Division, Department of Agriculture for Northern Ireland.
Appl Radiat Isot. 1993 Jan-Feb;44(1-2):433-7. doi: 10.1016/0969-8043(93)90262-9.
This paper examines the potential of ESR spectroscopy to determine if Norway lobsters have been irradiated. Ninety samples, each containing 3 whole Norway lobsters, were prepared, thirty were used as controls while the remaining sixty were given irradiation doses of approximately either 1 or 3 kGy. Following irradiation the samples were stored at 1 degree C for 0, 7, 14, 21 or 28 d. After each storage period the cuticle of the tail, carapace, claws and walking legs was removed, freeze-dried and ground prior to analysis using ESR spectroscopy. The control spectra were subtracted from their respective irradiated spectra thereby leaving the radiation-induced signal. Peak heights of the signals were measured. The ESR signals derived from the different components of the exoskeleton were similar in shape and varied only in their intensities. The claw samples gave the most intense signal while that from the walking legs was the weakest. There was a significant decay in the signal intensity over the storage period with the signal derived from cuticle of the claws showing the greatest diminution (44%) and that of the tail the least (17%). The signal intensities of the walking legs and carapace decreased by 22% and 30% respectively. In conclusion ESR spectroscopy is a useful technique for the qualitative detection of irradiated Norway lobster and shows considerable potential for quantification of dose received.
本文研究了电子自旋共振光谱法用于确定挪威龙虾是否受到辐照的潜力。制备了90个样本，每个样本包含3只完整的挪威龙虾，其中30个用作对照，其余60个给予大约1或3千戈瑞的辐照剂量。辐照后，样本在1摄氏度下储存0、7、14、21或28天。在每个储存期后，将尾部、头胸甲、爪子和步足的角质层去除，冷冻干燥并研磨，然后使用电子自旋共振光谱法进行分析。从各自的辐照光谱中减去对照光谱，从而得到辐射诱导信号。测量信号的峰值高度。来自外骨骼不同成分的电子自旋共振信号形状相似，仅强度不同。爪子样本的信号最强，而步足的信号最弱。在储存期间，信号强度有显著衰减，爪子角质层的信号衰减最大（44%），尾部的信号衰减最小（17%）。步足和头胸甲的信号强度分别下降了22%和30%。总之，电子自旋共振光谱法是一种用于定性检测辐照挪威龙虾的有用技术，并且在量化所接受剂量方面显示出相当大的潜力。
