链霉菌属和棒状杆菌属中氯霉素乙酰转移酶及氯霉素-3-乙酸酯酶的分布情况

Distribution of chloramphenicol acetyltransferase and chloramphenicol-3-acetate esterase among Streptomyces and Corynebacterium.

作者信息

Nakano H, Matsuhashi Y, Takeuchi T, Umezawa H

出版信息

J Antibiot (Tokyo). 1977 Jan;30(1):76-82. doi: 10.7164/antibiotics.30.76.

DOI:10.7164/antibiotics.30.76

Abstract

Chloramphenicol-3-acetate esterase activity was detected in cell-free extracts of strains of Streptomyces venezuela, Streptomyces sp. and Streptosporangium viridogriseum var. kofuense which produced chloramphenicol and also Corynebacterium hydrocarboclastus which produced chloramphenicol analogs (corynecins). None of the cell-free extracts of chloramphenicol- or corynecin-producing strains possessed chloramphenicol acetyltransferase activity under conditions which avoided the influenced of the esterase activity. Among 20 strains examined that did not produce chloramphenicol, chloramphenicol acetyltransferase was detected in cell-free extracts of one strain of Streptomyces coelicolor Müller and one strain of S. fradiae ISP5063.

摘要

在委内瑞拉链霉菌、链霉菌属和绿灰链孢囊菌小府变种的无细胞提取物中检测到氯霉素-3-乙酸酯酶活性，这些菌株能产生氯霉素，还有能产生氯霉素类似物（棒杆菌素）的解烃棒状杆菌。在避免酯酶活性影响的条件下，产氯霉素或产棒杆菌素的菌株的无细胞提取物均不具有氯霉素乙酰转移酶活性。在所检测的20株不产氯霉素的菌株中，在天蓝色链霉菌穆勒氏变种的一个菌株和弗氏链霉菌ISP5063的一个菌株的无细胞提取物中检测到了氯霉素乙酰转移酶。