O'Flaherty J T, Cordes J, Redman J, Thomas M J
Department of Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27157.
Biochem Biophys Res Commun. 1993 Apr 15;192(1):129-34. doi: 10.1006/bbrc.1993.1391.
5-Oxo-eicosatetraenoate (5-oxoETE), a newly defined arachidonate metabolite, resembled 5-hydroxyeicosatetraenoate (5-HETE) in stimulating neutrophils to mobilize Ca2+ an in promoting PMN degranulation responses to other agents. It was, however, 10-fold stronger than 5-HETE and, like leukotriene (LT) B4, had intrinsic PMN degranulating effects. Nonetheless, 5-oxoETE and 5-HETE desensitized PMN to themselves or each other but not to LTB4; LTB4 desensitized to itself but not to 5-oxoETE or 5-HETE; and an antagonist blocked LTB4 but not 5-oxoETE or 5-HETE. 5-OxoETE and 5-HETE thus induce diverse PMN responses using a shared, down-regulatable, and receptor-like mechanism that does not involve LTB4 receptors; 5-oxoETE is the preferred natural agonist for this mechanism.
5-氧代二十碳四烯酸酯（5-oxoETE）是一种新定义的花生四烯酸代谢产物，在刺激中性粒细胞动员Ca2+以及促进多形核白细胞（PMN）对其他介质的脱颗粒反应方面，类似于5-羟基二十碳四烯酸酯（5-HETE）。然而，它的作用强度比5-HETE强10倍，并且与白三烯（LT）B4一样，具有内在的PMN脱颗粒作用。尽管如此，5-oxoETE和5-HETE会使PMN对自身或彼此产生脱敏作用，但对LTB4不会；LTB4对自身产生脱敏作用，但对5-oxoETE或5-HETE不会；并且一种拮抗剂可阻断LTB4，但对5-oxoETE或5-HETE无效。因此，5-oxoETE和5-HETE利用一种不涉及LTB4受体的共同、可下调且类似受体的机制诱导不同的PMN反应；5-oxoETE是该机制的首选天然激动剂。
