Mujumdar M P, Mitra C K
Centre for Atmospheric Sciences, Indian Institute of Technology, New Delhi.
Comput Appl Biosci. 1993 Apr;9(2):227-30.
A computer simulation of the electrogenic nature of the membrane-bound Na+, K(+)-ATPase is presented. The model involves coupling two simulation systems for passive and active transports, using a minimum of empirical parameters, and studies the contribution of the pump to the membrane potential. The simulation results indicate that electrogenic active transport accelerates the restoration of the resting electrochemical gradients and contributes approximately 0.44-1.1 mV to the resting potential of the membrane, depending on the Na:K coupling ratio. The effect of membrane potential and the physical positioning of the enzyme from the passive transporting channel on the enzyme function is also presented. The validity of the model is checked by comparing our results with reported literature values.
本文介绍了膜结合型钠钾ATP酶电生性的计算机模拟。该模型通过最少的经验参数耦合了被动运输和主动运输的两个模拟系统，并研究了泵对膜电位的贡献。模拟结果表明，电生性主动运输加速了静息电化学梯度的恢复，根据钠钾耦合比，对膜的静息电位贡献约为0.44 - 1.1 mV。还介绍了膜电位以及酶相对于被动运输通道的物理位置对酶功能的影响。通过将我们的结果与已发表文献值进行比较，检验了该模型的有效性。
