Enomoto M, Leboy P S, Menko A S, Boettiger D
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6076.
Exp Cell Res. 1993 Apr;205(2):276-85. doi: 10.1006/excr.1993.1087.
Chondrocytes isolated from the cephalic region of sterna from 14-day-old chick embryos used beta 1 integrins and required either Mg2+ or Mn2+ for attachment to plates coated with type I collagen, type II collagen, and fibronectin. beta 1 integrin was concentrated in adhesion plaques of the chondrocytes plated on type I collagen, type II collagen, and fibronectin substrates. Chondrocytes expressed at least 3 alpha-subunits, including alpha 3, alpha 5, and putative alpha 2. alpha 5, but not alpha 3, had a higher molecular weight in chondrocytes than in fibroblasts. Levels of alpha 3 and alpha 5 were about 25-30% of that in fibroblasts. When the chondrocytes were cultured in the presence of ascorbate in suspension, the cells aggregated into clusters. This aggregation was dependent on beta 1 integrin and type II collagen.
从14日龄鸡胚胸骨头部区域分离出的软骨细胞使用β1整合素，并且在附着于涂有I型胶原、II型胶原和纤连蛋白的平板时需要Mg2+或Mn2+。β1整合素集中在接种于I型胶原、II型胶原和纤连蛋白底物上的软骨细胞的黏着斑中。软骨细胞表达至少3种α亚基，包括α3、α5和假定的α2。α5而非α3在软骨细胞中的分子量高于成纤维细胞。α3和α5的水平约为成纤维细胞中的25%-30%。当软骨细胞在抗坏血酸存在下悬浮培养时，细胞聚集成团簇。这种聚集依赖于β1整合素和II型胶原。
