[利用合成肽抗体在体内鉴定与18型人乳头瘤病毒（HPV）增强子相互作用的YB-1蛋白]

[In vivo identification of YB-1 protein, interacting with the enhancer of human papillomavirus (HPV) type 18, using antibodies to a synthetic peptide].

作者信息

Spitkovskiĭ D D, Roĭer G D, Mazurenko N N, Mikhaleva I I, Prudchenko I A, Korbukh I A, Sukhova N M, Kiselev F L

出版信息

Mol Biol (Mosk). 1993 Jan-Feb;27(1):81-91.

Abstract

Enhancer sequences of human papilloma virus (HPV) type 18 were used for screening of HeLa cells cDNA library in lambda gt11 using the protein binding method. Clones with YB I gene homology sequences were isolated. This gene is coding the protein which binds the regulatory region of Y gene of main histocompatibility complex (HLA 11). The YB I transcripts were revealed in all tested samples of cervical carcinomas. To analyze the protein the rabbit antibodies were produced to synthetic peptide, which corresponds to the most hydrophilic region of the protein. This antipeptide serum allowed to identify the nuclear 42K protein in HeLa cells as well as in normal fibroblasts.

摘要

人乳头瘤病毒18型（HPV-18）的增强子序列用于通过蛋白质结合法筛选λgt11载体中的HeLa细胞cDNA文库。分离出具有YB I基因同源序列的克隆。该基因编码与主要组织相容性复合体（HLA 11）Y基因调控区结合的蛋白质。在所有检测的宫颈癌样本中均检测到YB I转录本。为了分析该蛋白质，制备了针对合成肽的兔抗体，该合成肽对应于该蛋白质最亲水的区域。这种抗肽血清可用于鉴定HeLa细胞以及正常成纤维细胞中的核42K蛋白。

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