Haneda M, Kikkawa R, Koya D, Sakamoto K, Nakanishi S, Matsuda Y, Shigeta Y
Third Department of Medicine, Shiga University of Medical Science, Japan.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):642-8. doi: 10.1006/bbrc.1993.1463.
The mechanism of anti-proliferative action of atrial natriuretic peptide (ANP) was examined in cultured mesangial cells. HS-142-1, a selective antagonist for ANP biological receptors, inhibited ANP-induced cellular cGMP accumulation and prevented ANP-induced inhibition of both serum-stimulated incorporation of 3H-thymidine and cell proliferation. Des-[Gln18, Ser19, Gly20, Leu21, Gly22]-ANP4-23-NH2 (C-ANP) failed to inhibit 3H-thymidine incorporation. These results indicate that anti-proliferative action of ANP is mediated by the biological receptors in cultured mesangial cells.
在培养的系膜细胞中研究了心房利钠肽（ANP）的抗增殖作用机制。HS-142-1是一种ANP生物受体的选择性拮抗剂，它抑制ANP诱导的细胞cGMP积累，并阻止ANP诱导的对血清刺激的3H-胸腺嘧啶掺入和细胞增殖的抑制作用。去-[谷氨酰胺18，丝氨酸19，甘氨酸20，亮氨酸21，甘氨酸22]-ANP4-23-NH2（C-ANP）未能抑制3H-胸腺嘧啶掺入。这些结果表明，ANP的抗增殖作用是由培养的系膜细胞中的生物受体介导的。
