高氯酸盐诱导的核糖核酸酶A变性：对可能的折叠中间体的研究。

Perchlorate-induced denaturation of ribonuclease A: investigation of possible folding intermediates.

作者信息

Scholtz J M, Baldwin R L

机构信息

Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.

出版信息

Biochemistry. 1993 May 4;32(17):4604-8. doi: 10.1021/bi00068a017.

DOI:10.1021/bi00068a017

PMID:8387338

Abstract

Perchlorate-denatured ribonuclease A (PDR) is known to show a circular dichroism (CD) spectrum suggestive of substantial secondary structure. Thus, PDR may be a molten globule form of ribonuclease A. We find that any secondary structure present in PDR does not provide measurable protection against amide proton exchange, and PDR does not belong to the class of structured molten globules. CD spectra of short peptides show that the perchlorate anion affects these spectra in a way that could be mistaken for secondary structure formation; thus, caution must be used in interpreting CD spectra of peptides and proteins taken in perchlorate solutions.

摘要

高氯酸盐变性核糖核酸酶A（PDR）已知呈现出暗示大量二级结构的圆二色性（CD）光谱。因此，PDR可能是核糖核酸酶A的一种熔球态形式。我们发现PDR中存在的任何二级结构都不能为酰胺质子交换提供可测量的保护，并且PDR不属于结构化熔球的类别。短肽的CD光谱表明，高氯酸根阴离子以一种可能被误认为是二级结构形成的方式影响这些光谱；因此，在解释高氯酸盐溶液中肽和蛋白质的CD光谱时必须谨慎。