Chiba H, Sawada N, Ono T, Ishii S, Mori M
Department of Pathology, Sapporo Medical College.
Jpn J Cancer Res. 1993 Mar;84(3):290-7. doi: 10.1111/j.1349-7006.1993.tb02869.x.
We established a human osteoblastic cell line immortalized by simian virus 40 (SV40) in vitro, and designated it SV-HFO. Immunocytochemically, the cells were positive for SV40 large T-antigen, vimentin and osteocalcin, but negative for keratin and epithelial membrane antigen. The cells had characteristic morphologic and ultrastructural features of osteoblasts, produced alkaline phosphatase, and synthesized osteocalcin, the levels of which were elevated by treatment of the cells with 1a,25-dihydroxyvitamin D3. The cells proliferated and showed such osteoblastic properties even under serum-free conditions. The cells grew in soft agar, but did not form tumors when transplanted into athymic nude mice. Karyotypic analysis by the Q-banding technique showed that these cells were of human origin. The SV-HFO cell line is expected to serve as a suitable model for studying metabolism and carcinogenesis in human bone.
我们在体外建立了一种由猿猴病毒40（SV40）永生化的人成骨细胞系，并将其命名为SV-HFO。免疫细胞化学检测显示，这些细胞对SV40大T抗原、波形蛋白和骨钙素呈阳性反应，但对角蛋白和上皮膜抗原呈阴性反应。这些细胞具有成骨细胞特有的形态学和超微结构特征，能产生碱性磷酸酶，并合成骨钙素，用1α,25-二羟基维生素D3处理细胞后，骨钙素水平升高。即使在无血清条件下，这些细胞仍能增殖并表现出成骨细胞特性。这些细胞能在软琼脂中生长，但移植到无胸腺裸鼠体内时不形成肿瘤。通过Q带技术进行的核型分析表明，这些细胞来源于人类。SV-HFO细胞系有望成为研究人类骨骼代谢和致癌作用的合适模型。
